Abstract
Highly reproducible and simple protocol for cotton somatic embryogenesis is described here by using different concentrations of maltose, glucose, sucrose and fructose. Maltose (30 g/l) is the best carbon source for embryogenic callus induction and glucose (30 g/l) was suitable for induction, maturation of embryoids and plant regeneration. Creamy white embryogenic calli of hypocotyl explants were formed on medium containing MS basal salts, myo-inositol (100 mg/l), thiamine HCI (0.3 mg/l), picloram (0.3 mg/l), Kin (0.1 mg/l) and maltose (30 g/l). During embryo induction and maturation, accelerated growth was observed in liquid medium containing NH3NO4 (1 g/l), picloram (2.0 mg/l), 2 ip (0.2 mg/l), Kin (0.1 mg/l) and glucose (30 g/l). Before embryoid induction, large clumps of embryogenic tissue were formed. These tissues only produced viable embryoids. Completely matured somatic embryos were germinated successfully on the medium fortified with MS salts, myo-inositol (50 mg/l), thiamine HCl (0.2 mg/l), GA3 (0.2 mg/l), BA (1.0 mg/l) and glucose (30 g/l). Compared with earlier reports, 65% of somatic embryo germination was observed. The abnormal embryo formation was highly reduced by using glucose (30 g/l) compared to other carbon sources. The regenerated plantlets were fertile but smaller in height than the seed derived control plants.
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Claims 11
Claim · model-written summary
Maltose at 30 g/l was the best carbon source for embryogenic callus induction.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- Maltose (30 g/l)e0
- Outcome
- embryogenic callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Maltose (30 g/l) is the best carbon source for embryogenic callus induction”
Claim · model-written summary
Glucose at 30 g/l was suitable for embryoid induction, maturation and plant regeneration.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- glucose (30 g/l)e0
- Outcome
- induction, maturation of embryoids and plant regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“glucose (30 g/l) was suitable for induction, maturation of embryoids and plant regeneration”
Claim · model-written summary
Hypocotyl explants formed creamy white embryogenic calli on the specified maltose medium.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- hypocotyl explantse0
- Treatment
- medium containing MS basal salts, myo-inositol (100 mg/l), thiamine HCI (0.3 mg/l), picloram (0.3 mg/l), Kin (0.1 mg/l) and maltose (30 g/l)e0
- Outcome
- Creamy white embryogenic callie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Creamy white embryogenic calli of hypocotyl explants were formed on medium containing MS basal salts, myo-inositol (100 mg/l), thiamine HCI (0.3 mg/l), picloram (0.3 mg/l), Kin (0.1 mg/l) and maltose (30 g/l)”
Claim · model-written summary
Embryos showed accelerated growth in the specified glucose liquid medium.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- liquid medium containing NH3NO4 (1 g/l), picloram (2.0 mg/l), 2 ip (0.2 mg/l), Kin (0.1 mg/l) and glucose (30 g/l)e0
- Outcome
- growthe0
- Direction
- acceleratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“accelerated growth was observed in liquid medium containing NH3NO4 (1 g/l), picloram (2.0 mg/l), 2 ip (0.2 mg/l), Kin (0.1 mg/l) and glucose (30 g/l)”
Claim · model-written summary
Large clumps of embryogenic tissue formed before embryoid induction.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- embryogenic tissuee0
- Outcome
- large clumps of embryogenic tissue were formede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Before embryoid induction, large clumps of embryogenic tissue were formed”
Claim · model-written summary
Only the large clumps of embryogenic tissue produced viable embryoids.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- embryogenic tissuee0
- Outcome
- viable embryoidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“large clumps of embryogenic tissue were formed. These tissues only produced viable embryoids”
Claim · model-written summary
Mature somatic embryos germinated on the specified glucose medium.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- Completely matured somatic embryose0
- Treatment
- medium fortified with MS salts, myo-inositol (50 mg/l), thiamine HCl (0.2 mg/l), GA3 (0.2 mg/l), BA (1.0 mg/l) and glucose (30 g/l)e0
- Outcome
- germinated successfullye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Completely matured somatic embryos were germinated successfully on the medium fortified with MS salts, myo-inositol (50 mg/l), thiamine HCl (0.2 mg/l), GA3 (0.2 mg/l), BA (1.0 mg/l) and glucose (30 g/l)”
Claim · model-written summary
Somatic embryo germination was 65% compared with earlier reports.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- somatic embryo germinatione0
- Value
- 65%e0
- Comparator
- earlier reportse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Compared with earlier reports, 65% of somatic embryo germination was observed”
Claim · model-written summary
Glucose at 30 g/l greatly reduced abnormal embryo formation relative to other carbon sources.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- glucose (30 g/l)e0
- Outcome
- abnormal embryo formatione0
- Direction
- highly reducede0
- Comparator
- other carbon sourcese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“The abnormal embryo formation was highly reduced by using glucose (30 g/l) compared to other carbon sources”
Claim · model-written summary
Regenerated plantlets were fertile.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- regenerated plantletse0
- Outcome
- fertilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“The regenerated plantlets were fertile but smaller in height than the seed derived control plants”
Claim · model-written summary
Regenerated plantlets were shorter than seed derived control plants.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- regenerated plantletse0
- Outcome
- heighte0
- Direction
- smallere0
- Comparator
- seed derived control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“The regenerated plantlets were fertile but smaller in height than the seed derived control plants”
The same questions in other papers
maltose · growth · Gossypium hirsutum (cotton)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.