Abstract
Tobacco hexaploid plants were obtained successfully by crossingYunyan87 octoploids(2n=8x=96) with L-8 tetraploids, with octoploids as maternal parent. Fertility was studied with traits of flower and pollen, pollen germination in vitro and cross experiment. Results showed that: ?ower buds and anthers of hexaploid tobaccos were bigger than those of tetraploid tobaccos, with less amount of pollen. Under in vitro conditions, pollen germination rate of hexaploids was only 9.78%, significantly lower than that of tetraploid. Only a very small amount of hexaploid pollen could germinate on Yunyan87 stigma. There was no fruit when hexaploid as male parent while only some mature seeds were harvested when hexaploid as maternal parent. Germination rate of these seeds was only 21.2%. Our data suggested that hexaploid tobacco plants was male sterile and could be used as female parent in future breeding and genetic study.
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Claims 10
Claim · model-written summary
Crossing Yunyan87 octoploids with L-8 tetraploids produced hexaploid tobacco plants.
- Taxon
- Tobacco
Nicotiana tabacume0 - Material
- hexaploid plantse0
- Outcome
- hexaploid plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco hexaploid plants were obtained successfully by crossingYunyan87 octoploids(2n=8x=96) with L-8 tetraploids, with octoploids as maternal parent”
Claim · model-written summary
Hexaploid tobacco had bigger flower buds and anthers than tetraploid tobacco.
- Taxon
- tobaccosNicotiana tabacume0
- Material
- hexaploide0
- Outcome
- ?ower buds and antherse0
- Direction
- biggere0
- Comparator
- tetraploid tobaccose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“?ower buds and anthers of hexaploid tobaccos were bigger than those of tetraploid tobaccos”
Claim · model-written summary
Hexaploid tobacco produced less pollen than tetraploid tobacco.
- Taxon
- tobaccosNicotiana tabacume0
- Material
- hexaploide0
- Outcome
- amount of pollene0
- Direction
- lesse0
- Comparator
- tetraploid tobaccose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“?ower buds and anthers of hexaploid tobaccos were bigger than those of tetraploid tobaccos, with less amount of pollen”
Claim · model-written summary
In vitro hexaploid pollen germination was 9.78%, significantly below tetraploid pollen germination.
- Material
- hexaploidse0
- Outcome
- pollen germination ratee0
- Direction
- lowere0
- Value
- 9.78%e0
- Comparator
- tetraploide0
- Setting
- in vitro conditionse0
Not reportedTaxon
Verbatim
e0“Under in vitro conditions, pollen germination rate of hexaploids was only 9.78%, significantly lower than that of tetraploid”
Claim · model-written summary
Very little hexaploid pollen germinated on Yunyan87 stigma.
Not reportedTaxon
Verbatim
e0“Only a very small amount of hexaploid pollen could germinate on Yunyan87 stigma”
Claim · model-written summary
Using hexaploid tobacco as male parent produced no fruit.
Not reportedTaxon
Verbatim
e0“There was no fruit when hexaploid as male parent”
Claim · model-written summary
Using hexaploid tobacco as maternal parent produced some mature seeds.
Not reportedTaxon
Verbatim
e0“only some mature seeds were harvested when hexaploid as maternal parent”
Claim · model-written summary
Seed germination from hexaploid maternal crosses was 21.2%.
Not reportedTaxon
Verbatim
e0“Germination rate of these seeds was only 21.2%”
Claim · model-written summary
The authors interpreted hexaploid tobacco as male sterile.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- hexaploide0
- Outcome
- male sterilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hexaploid tobacco plants was male sterile”
Claim · model-written summary
The authors proposed using hexaploid tobacco as a female parent in breeding and genetic studies.
- Outcome
- female parent in future breeding and genetic studye0
Not reportedTaxon
Verbatim
e0“could be used as female parent in future breeding and genetic study”