Abstract
Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations.More and better calli were induced from cotyledons than from hypocotyls.NAA was better than 2,4-D and 2,4-D was better than IBA in callus induction.The optimum medium for callus formation from cotyledons and hypocotyls were MS + 6-BA 3 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar.Subculture promoted the formation of calli and the optimum subculture was MS + 6-BA 6 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for cotyledon explants and MS + 6-BA 4 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for hypocotyl explants.
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Claims 7
Claim · model-written summary
Radish cotyledons gave more and better calli than hypocotyls.
- Taxon
- radish
Raphanus sativuse0 - Organ / stage
- cotyledonse1
- Outcome
- callie1
- Direction
- More and bettere1
- Comparator
- hypocotylse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e1“More and better calli were induced from cotyledons than from hypocotyls”
Claim · model-written summary
NAA gave better callus induction than 2,4-D in radish explants.
- Taxon
- radishRaphanus sativuse0
- Treatment
- NAAe2
- Outcome
- callus inductione2
- Direction
- bettere2
- Comparator
- 2,4-De2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e2“NAA was better than 2,4-D and 2,4-D was better than IBA in callus induction”
Claim · model-written summary
2,4-D gave better callus induction than IBA in radish explants.
- Taxon
- radishRaphanus sativuse0
- Treatment
- 2,4-De2
- Outcome
- callus inductione2
- Direction
- bettere2
- Comparator
- IBAe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e2“NAA was better than 2,4-D and 2,4-D was better than IBA in callus induction”
Claim · model-written summary
MS medium with 6-BA 3 mg/L and NAA 0.5 mg/L was the optimum medium for callus formation from radish cotyledons and hypocotyls.
- Taxon
- radishRaphanus sativuse0
- Organ / stage
- cotyledons and hypocotylse3
- Treatment
- MS + 6-BA 3 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agare3
- Outcome
- optimum medium for callus formatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e3“The optimum medium for callus formation from cotyledons and hypocotyls were MS + 6-BA 3 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar”
Claim · model-written summary
Subculture promoted callus formation in radish explants.
- Taxon
- radishRaphanus sativuse0
- Treatment
- Subculturee4
- Outcome
- formation of callie4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e4“Subculture promoted the formation of calli and the optimum subculture was MS + 6-BA 6 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for cotyledon explants and MS + 6-BA 4 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for hypocotyl explants”
Claim · model-written summary
The optimum subculture medium for radish cotyledon explants was MS with 6-BA 6 mg/L and NAA 0.5 mg/L.
- Taxon
- radishRaphanus sativuse0
- Organ / stage
- cotyledon explantse4
- Treatment
- MS + 6-BA 6 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agare4
- Outcome
- optimum subculturee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e4“Subculture promoted the formation of calli and the optimum subculture was MS + 6-BA 6 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for cotyledon explants and MS + 6-BA 4 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for hypocotyl explants”
Claim · model-written summary
The optimum subculture medium for radish hypocotyl explants was MS with 6-BA 4 mg/L and NAA 0.5 mg/L.
- Taxon
- radishRaphanus sativuse0
- Organ / stage
- hypocotyl explantse4
- Treatment
- MS + 6-BA 4 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agare4
- Outcome
- optimum subculturee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyls and cotyledons of radish(Raphanus sativus L.) were used as explants and cultured on MS media supplemented with different combinations of hormones at different concentrations”
e4“Subculture promoted the formation of calli and the optimum subculture was MS + 6-BA 6 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for cotyledon explants and MS + 6-BA 4 mg/L + NAA 0.5 mg/L + 3% sucrose + 0.8% agar for hypocotyl explants”
The same questions in other papers
2,4-D · growth · Raphanus sativus (radish)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
1-naphthaleneacetic acid · growth · Raphanus sativus (radish)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.