Abstract
042BM noeA was obtained by PCR. It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%. In addition, it was found that this protein shares significant homology with the SAM-dependent methyltransferase of Mesorhizobium sp. BNC1 (32% similarity), and the similarity of its 303-362 region to the 160- 220 region of Ll11 methyltransferases of E. coli (PrmA) is 41%. Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu. However, this mutant has no significant change in ability to nodulate cultivars of Huanghou and Zahua. Hence, noeA is involved in alfalfa cultivar-specific nodulation.
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Claims 10
Claim · model-written summary
The 042BM noeA sequence was 99% identical to the S. meliloti 1021 sequence.
- Taxon
- alfalfa
Medicago sativae1 - Gene
- noeAt0
- Outcome
- identicale0
- Value
- 99%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%.”
t0“noeA from Sinorhizobium meliloti 042BM”
e1“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
Claim · model-written summary
NoeA similarity between 042BM and S. meliloti 1021 was 97%.
Not reportedTaxon
Verbatim
e0“It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%.”
Claim · model-written summary
NoeA shared 32% similarity with a Mesorhizobium methyltransferase.
Not reportedTaxon
Verbatim
e0“this protein shares significant homology with the SAM-dependent methyltransferase of Mesorhizobium sp. BNC1 (32% similarity)”
Claim · model-written summary
NoeA residues 303-362 had 41% similarity to an E. coli methyltransferase region.
Not reportedTaxon
Verbatim
e0“the similarity of its 303-362 region to the 160- 220 region of Ll11 methyltransferases of E. coli (PrmA) is 41%.”
Claim · model-written summary
The noeA deletion mutant increased nodule number in five alfalfa cultivars.
- Taxon
- alfalfaMedicago sativae0
- Material
- Putong Zihua, Baoding, Ningxia, Baifa and Aohane0
- Treatment
- noeA deletion mutant 042BMA-Kme0
- Interactor
- 042BMA-Kme0symbiont
- Gene
- noeAe0
- Outcome
- number of nodulee0
- Direction
- increasee0
- Comparator
- 042BMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
Claim · model-written summary
The noeA deletion mutant increased nodule fresh weight in five alfalfa cultivars.
- Taxon
- alfalfaMedicago sativae0
- Material
- Putong Zihua, Baoding, Ningxia, Baifa and Aohane0
- Treatment
- noeA deletion mutant 042BMA-Kme0
- Outcome
- fresh weight of nodulee0
- Direction
- increasee0
- Comparator
- 042BMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
Claim · model-written summary
The noeA deletion mutant increased plant top dry weight in five alfalfa cultivars.
- Taxon
- alfalfaMedicago sativae0
- Material
- Putong Zihua, Baoding, Ningxia, Baifa and Aohane0
- Treatment
- noeA deletion mutant 042BMA-Kme0
- Outcome
- plant top dry weighte0
- Direction
- increasee0
- Comparator
- 042BMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
Claim · model-written summary
The noeA deletion mutant decreased the three measured traits on Milu alfalfa.
- Taxon
- alfalfaMedicago sativae0
- Material
- Milue0
- Treatment
- noeA deletion mutant 042BMA-Kme0
- Outcome
- number of nodule, fresh weight of nodule and plant top dry weighte0
- Direction
- decreasee0
- Comparator
- 042BMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
Claim · model-written summary
The mutant showed no significant change in nodulation of Huanghou and Zahua.
- Taxon
- alfalfaMedicago sativat0
- Material
- Huanghou and Zahuae0
- Treatment
- this mutante0
- Outcome
- ability to nodulatee0
- Direction
- no significant changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this mutant has no significant change in ability to nodulate cultivars of Huanghou and Zahua.”
t0“alfalfa cultivars of Putong Zihua”
Claim · model-written summary
The authors conclude that noeA contributes to cultivar-specific alfalfa nodulation.
- Taxon
- alfalfaMedicago sativae0
- Gene
- noeAe0
- Outcome
- cultivar-specific nodulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hence, noeA is involved in alfalfa cultivar-specific nodulation.”