Abstract
Objective To clone the gene of the toxic A chain of mistletoe protein with N-glyco-sidase activity.Methods Ammonium sulfate precipitation, affinity chromatography and cation exchange chromatography were employed to extract, isolate and purify the proteins with galactose-binding specificity from mistletoe [ Viscum coloratum (Komar.] Nakai) in Jilin province.After SDS-PAGE, the polypeptide bands were electroblotted onto polyvinylidene difluoride membrane for sequence analysis.The N-terminal sequences of mistletoe proteins were determined by automated Ed-man degradation.The cDNA fragment of A chain of one mistletoe protein was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with the primers based on the N-terminal sequence results.Results Two new toxic proteins named CM-1, CM-2 were purified from V.coloratum.The search of Genbank showed that the deduced amino acid sequence of A chain of CM-1 was highly homologous with the A chains of mistletoe proteins in V.album.Conclusion The cDNA fragment encoding A chain of CM-1 was successfully cloned.
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Claims 4
Claim · model-written summary
CM-1 was purified from V. coloratum.
- Taxon
- V.coloratum
Viscum coloratume0 - Gene
- CM-1e0
- Outcome
- CM-1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two new toxic proteins named CM-1, CM-2 were purified from V.coloratum”
Claim · model-written summary
CM-2 was purified from V. coloratum.
- Taxon
- V.coloratumViscum coloratume0
- Gene
- CM-2e0
- Outcome
- CM-2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two new toxic proteins named CM-1, CM-2 were purified from V.coloratum”
Claim · model-written summary
The deduced CM-1 A-chain amino acid sequence was highly homologous to V. album mistletoe protein A chains.
- Taxon
- V.coloratumViscum coloratume1
- Gene
- A chain of CM-1e0
- Outcome
- highly homologous with the A chains of mistletoe proteins in V.albume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deduced amino acid sequence of A chain of CM-1 was highly homologous with the A chains of mistletoe proteins in V.album”
e1“from V.coloratum”
Claim · model-written summary
The cDNA fragment encoding the CM-1 A chain was cloned.
- Taxon
- V.coloratumViscum coloratume1
- Gene
- A chain of CM-1e0
- Outcome
- cDNA fragment encoding A chain of CM-1e0
- Value
- CM-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cDNA fragment encoding A chain of CM-1 was successfully cloned”
e1“from V.coloratum”