Antibiotic-resistance tag of Stremptomyces roseoflavus Men-myco-93-63,its colonization and effects on plant growth-promoting

Abstract

In order to develop the antibiotic-resistance tag of Stremptomyces roseoflavus Men-myco-93-63,eight kinds of antibiotics or microbicides were tested,including procymidone,carbendazim,thiophanate-methyl,rifabutin,penicillin,streptomycin,tylosin,potessium,and dichromate potassium.It was determined that potessium dichromate potassium with the concentration of 60 mg/L and penicillin with the concentration of 200 mg/L are the desirable antibiotic-resistance tag for the detection of Men-myco-93-63.Using developed tag,with the observation of colony morphology and the sequence analysis of 16S rDNA,colonizing dynamics of the strain around the tomato rhizosphere and their effects on the plants growth were evaluated.Results show that Men-myco-93-63 can colonize around the tomato rhizosphere.The amount of colonization reached the peak at the 15th day after inoculation,and then declined step by step.There was no Men-myco-93-63 detected 30 days after inoculation.Moreover,it was found that Men-myco-93-63 showed growth-promoting effect on tomato seedling,if maturing the mixture of Men-myco-93-63 solid fermentation with soil in proportion of 1:400.

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