Abstract
Effects of different culture conditions including different concentration of sucrose and agar added in media,different pH values, culture temperature and light intension on rooting in vitro of test-tube plantlets of Vitis vinifera L.were carried out.The results showed that the optimum concentration of sucrose for rooting was 15～20 g/L.pH value had significant effects on root number,root diameter,root length and root-shoot ratio of test-tube plantlets cultured with different media,pH value increased,root number and average root length decreased,root diameter and root-shoot ratio increased.Concentration of agar significantly affected the rooting of test-tube plantlets,the high agar concentration was the later root primordium produced,meanwhile,as concentration of agar increased,average root length and root-shoot ratio decreased,average root diameter and root number increased.Root number showed no significant differences among three temperature treatments,while the temperature increased,average root diameter decreased a little,average root length and root-shoot ratio increased first,but when temperature reached to 35 ℃,they decreased.With the increasing of light intension,number,length and diameter of root and root-shoot ratio were all increased.
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Claims 10
Claim · model-written summary
The optimum sucrose concentration for rooting was 15–20 g/L.
Not reportedTaxon
Verbatim
e0“the optimum concentration of sucrose for rooting was 15～20 g/L”
Claim · model-written summary
Higher pH reduced root number and average root length.
Not reportedTaxon
Verbatim
e0“pH value increased,root number and average root length decreased”
Claim · model-written summary
Higher pH increased root diameter and root-shoot ratio.
Not reportedTaxon
Verbatim
e0“root diameter and root-shoot ratio increased”
Claim · model-written summary
Higher agar concentration delayed production of root primordia.
Not reportedTaxon
Verbatim
e0“the high agar concentration was the later root primordium produced”
Claim · model-written summary
Increasing agar concentration decreased average root length and root-shoot ratio.
- Treatment
- concentration of agare0
- Outcome
- average root length and root-shoot ratioe0
- Direction
- decreasede0
Not reportedTaxon
Verbatim
e0“as concentration of agar increased,average root length and root-shoot ratio decreased”
Claim · model-written summary
Increasing agar concentration increased average root diameter and root number.
Not reportedTaxon
Verbatim
e0“average root diameter and root number increased”
Claim · model-written summary
Root number did not differ significantly among three temperature treatments.
Not reportedTaxon
Verbatim
e0“Root number showed no significant differences among three temperature treatments”
Claim · model-written summary
Average root diameter decreased slightly as temperature increased.
Not reportedTaxon
Verbatim
e0“while the temperature increased,average root diameter decreased a little”
Claim · model-written summary
Average root length and root-shoot ratio initially increased with temperature, then decreased at 35 ℃.
Not reportedTaxonDirection
Verbatim
e0“average root length and root-shoot ratio increased first,but when temperature reached to 35 ℃,they decreased”
Claim · model-written summary
Increasing light intensity increased root number, length, diameter, and root-shoot ratio.
Not reportedTaxon
Verbatim
e0“With the increasing of light intension,number,length and diameter of root and root-shoot ratio were all increased”