Abstract
Expression analysis about wheat chitinase gene,in the different growing stages and different positions of different resistance cultivars after CCN inoculation,was carried by semi-Q RT-PCR,using wheat Actin gene as a control.The result showed that the quantity of chitinase gene expression obvious increased after 12 h inoculation,and there were no obvious differences between resistant and susceptible cultivars.After 36 h inoculation,the expression quantity in both cultivars increased obviously,and the quantity in resistant cultivars was more than susceptible cultivars,and in leaves was more than in roots.After 60 h inoculation,the gene expression quantity in resistant and susceptible cultivars was keeping a high level,and the former was more than the latter.The expression in susceptible cultivars after 84 h inoculation didn't have obvious difference from the ones without inoculation,but the expression quantity in resistant cultivars was still higher than those without inoculation.
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Claims 7
Claim · model-written summary
After 12 h of cereal cyst nematode inoculation, wheat chitinase gene Cht4 expression increased.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- 12 h inoculatione1
- Interactor
- cereal cyst nematode
.jpg?width=60)
Heterodera avenaet1pathogen - Gene
- Cht4t0
- Outcome
- chitinase gene expressione1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“the quantity of chitinase gene expression obvious increased after 12 h inoculation”
t0“wheat chitinase gene Cht4”
t1“cereal cyst nematode”
Claim · model-written summary
At 12 h after inoculation there was no obvious difference in chitinase gene expression between resistant and susceptible wheat cultivars.
- Taxon
- wheatTriticum aestivumt0
- Material
- resistant and susceptible cultivarse2
- Outcome
- differences between resistant and susceptible cultivarse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“there were no obvious differences between resistant and susceptible cultivars”
t0“wheat chitinase gene Cht4”
Claim · model-written summary
After 36 h of cereal cyst nematode inoculation, chitinase gene expression increased in both resistant and susceptible wheat cultivars.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- 36 h inoculatione3
- Interactor
- cereal cyst nematodeHeterodera avenaet1pathogen
- Gene
- Cht4t0
- Outcome
- expression quantitye3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“After 36 h inoculation,the expression quantity in both cultivars increased obviously”
t0“wheat chitinase gene Cht4”
t1“cereal cyst nematode”
Claim · model-written summary
At 36 h after inoculation, chitinase gene expression was higher in resistant than in susceptible wheat cultivars.
- Taxon
- wheatTriticum aestivumt0
- Material
- resistant cultivarse4
- Outcome
- quantitye4
- Direction
- moree4
- Comparator
- susceptible cultivarse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the quantity in resistant cultivars was more than susceptible cultivars”
t0“wheat chitinase gene Cht4”
Claim · model-written summary
At 36 h after inoculation, chitinase gene expression was higher in leaves than in roots of wheat.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- leavese5
- Outcome
- expression quantitye3
- Direction
- moree5
- Comparator
- rootse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“After 36 h inoculation,the expression quantity in both cultivars increased obviously”
e5“in leaves was more than in roots”
t0“wheat chitinase gene Cht4”
Claim · model-written summary
At 84 h after inoculation, chitinase gene expression in susceptible wheat cultivars did not differ from uninoculated plants.
- Taxon
- wheatTriticum aestivumt0
- Material
- susceptible cultivarse6
- Outcome
- expressione6
- Direction
- didn't have obvious differencee6
- Comparator
- the ones without inoculatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The expression in susceptible cultivars after 84 h inoculation didn't have obvious difference from the ones without inoculation”
t0“wheat chitinase gene Cht4”
Claim · model-written summary
At 84 h after inoculation, chitinase gene expression in resistant wheat cultivars remained higher than in uninoculated plants.
- Taxon
- wheatTriticum aestivumt0
- Material
- resistant cultivarse7
- Treatment
- inoculatione7
- Outcome
- expression quantitye7
- Direction
- highere7
- Comparator
- those without inoculatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“the expression quantity in resistant cultivars was still higher than those without inoculation”
t0“wheat chitinase gene Cht4”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.