Abstract
The effects of high temperature heat-shock treatment,sucrose concentrations,adding and replacing culture liquids,kinds of hormones and their concentrations ration on microspore embryo yield with two of the best head cabbage F1 hybrids were studied.The results showed that it was necessary for microspore embryogenesis to use 33℃ high-temperature heat-shock treatment for 24h.It could get the highest embryo yield when microspores were cultured in NLN-13,reaching 1.89 embryo yield per bud.The embryo yield was greatly improved by adding medium of low sucrose concentration(NLN-10) into the medium of high sucrose concentration(NLN-17) after 3 days cultured,embryoid was increased by 255.2% compared with microspores cultured consistently in the medium of NLN-13 sucrose concentration.Adding 0.1mg/L 6-BA and 0.05mg/LNAA into the medium of NLN-13 sucrose concentration could induce higher embryo yield.In conclusion,usage of some techniques can greatly improve embryo yield.
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Claims 4
Claim · model-written summary
A 33℃ heat shock for 24h was necessary for microspore embryogenesis.
- Taxon
- Cabbage
Brassica oleracea var. capitatat0 - Treatment
- 33℃ high-temperature heat-shock treatment for 24he0
- Outcome
- microspore embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was necessary for microspore embryogenesis to use 33℃ high-temperature heat-shock treatment for 24h.”
t0“Cabbage”
Claim · model-written summary
NLN-13 yielded the most embryos, at 1.89 per bud.
- Taxon
- CabbageBrassica oleracea var. capitatat0
- Treatment
- NLN-13e0
- Outcome
- embryo yielde0
- Direction
- higheste0
- Value
- 1.89 embryo yield per bude0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It could get the highest embryo yield when microspores were cultured in NLN-13,reaching 1.89 embryo yield per bud.”
t0“Cabbage”
Claim · model-written summary
Adding NLN-10 to NLN-17 after three days increased embryoids by 255.2% versus continued NLN-13 culture.
- Taxon
- CabbageBrassica oleracea var. capitatat0
- Treatment
- adding medium of low sucrose concentration(NLN-10) into the medium of high sucrose concentration(NLN-17)e0
- Outcome
- embryoide0
- Direction
- increasede0
- Value
- 255.2%e0
- Comparator
- microspores cultured consistently in the medium of NLN-13 sucrose concentratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryo yield was greatly improved by adding medium of low sucrose concentration(NLN-10) into the medium of high sucrose concentration(NLN-17) after 3 days cultured,embryoid was increased by 255.2% compared with microspores cultured consistently in the medium of NLN-13 sucrose concentration.”
t0“Cabbage”
Claim · model-written summary
Adding 6-BA and NAA to NLN-13 induced higher embryo yield.
- Taxon
- CabbageBrassica oleracea var. capitatat0
- Treatment
- Adding 0.1mg/L 6-BA and 0.05mg/LNAA into the medium of NLN-13 sucrose concentratione0
- Outcome
- embryo yielde0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding 0.1mg/L 6-BA and 0.05mg/LNAA into the medium of NLN-13 sucrose concentration could induce higher embryo yield.”
t0“Cabbage”
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- no effect
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- positive
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in vitro · yield · Brassica oleracea (wild cabbage)Positive and negative results
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- no effect
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9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.