Abstract
The regeneration process of the plants obtained from androgenetic embryos is a very important stage in obtaining homozygotic lines from anther cultures. The efficacy of regeneration depends above all on genotype. Another important factor influencing the regeneration process is the composition of the medium. Different authors recommend various modifications of the media used to regenerate plants from androgenetic embryos. Some researchers obtain whole plants on the regeneration media, while some others carry out this process in two stages: first regenerating shoots, and then stimulating root formation. In the present study, an effective method of plant regeneration from anther-derived embryos was used to secure sufficient plant material for creating homozygous lines. The experiments were carried out on two species: cabbage and carrots. The aim was to determine the effect of genotype and medium composition on the efficiency of the regeneration process. The genotype was the factor which had the greatest impact on plant regeneration from androgenic embryos in both cabbage and carrots. In cabbage, the highest number of shoots regenerated from embryos was obtained with 'Kamienna Glowa'. In carrots, the highest number of plants were formed with 'Feria F 1 ' and 'Narbonne F 1 '. When B5 medium (Gamborg et al., 1968) with 20 mg/l sucrose without hormones and and B5 with 20mg/l sucrose and 20 mg/l kinetine were used, shoot production was more intensive in cabbage. B5 with 20mg/l sucrose and 20 mg/l kinetine was useless for regenerating carrot embryos. In carrots secondary embryogenesis took place during the regeneration of embryos on media without hormones.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
In cabbage, the highest number of shoots regenerated from embryos was obtained with Kamienna Glowa.
- Taxon
- cabbage
Brassica oleracea var. capitatae1 - Material
- Kamienna Glowae1
- Outcome
- number of shoots regenerated from embryose1
- Direction
- higheste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In cabbage, the highest number of shoots regenerated from embryos was obtained with 'Kamienna Glowa'”
Claim · model-written summary
In carrots, the highest number of plants were formed with Feria F1 and Narbonne F1.
- Taxon
- carrots
Daucus carotae2 - Material
- Feria F 1e2
- Outcome
- number of plantse2
- Direction
- higheste2
- Qualifier
- Narbonne F 1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“In carrots, the highest number of plants were formed with 'Feria F 1 ' and 'Narbonne F 1 '”
Claim · model-written summary
In cabbage, B5 medium with 20 mg/l sucrose without hormones gave more intensive shoot production than B5 with 20 mg/l sucrose and 20 mg/l kinetin.
- Taxon
- cabbageBrassica oleracea var. capitatae3
- Treatment
- B5 medium (Gamborg et al., 1968) with 20 mg/l sucrose without hormonese3
- Outcome
- shoot productione3
- Direction
- more intensivee3
- Comparator
- B5 with 20mg/l sucrose and 20 mg/l kinetinee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“When B5 medium (Gamborg et al., 1968) with 20 mg/l sucrose without hormones and and B5 with 20mg/l sucrose and 20 mg/l kinetine were used, shoot production was more intensive in cabbage”
Claim · model-written summary
B5 medium with 20 mg/l sucrose and 20 mg/l kinetin was useless for regenerating carrot embryos.
- Taxon
- carrotDaucus carotae4
- Treatment
- B5 with 20mg/l sucrose and 20 mg/l kinetinee4
- Outcome
- regenerating carrot embryose4
- Qualifier
- uselesse4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“B5 with 20mg/l sucrose and 20 mg/l kinetine was useless for regenerating carrot embryos”
Claim · model-written summary
In carrots, secondary embryogenesis took place during embryo regeneration on media without hormones.
- Taxon
- carrotsDaucus carotae5
- Treatment
- media without hormonese5
- Outcome
- secondary embryogenesise5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“In carrots secondary embryogenesis took place during the regeneration of embryos on media without hormones”
Claim · model-written summary
Genotype had the greatest impact on plant regeneration from androgenic embryos in both cabbage and carrots.
- Taxon
- cabbage and carrotsBrassica oleracea var. capitataDaucus carotae6
- Treatment
- genotypee6
- Outcome
- plant regeneration from androgenic embryose6
- Direction
- greatest impacte6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The genotype was the factor which had the greatest impact on plant regeneration from androgenic embryos in both cabbage and carrots”
The same questions in other papers
sucrose · organism · Daucus carota (carrot)Results agree
1 paper in the same direction as this paper
- no effect
sucrose · yield · Brassica oleracea (wild cabbage)Results agree
2 papers in the same direction as this paper
- positive
- positive
class · yield · Brassica oleracea (wild cabbage)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
3 papers more in this group
See all 7 papers →in vitro · yield · Brassica oleracea (wild cabbage)Positive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.