Abstract
A practical method of detecting the content of Vitamin C in Fructus chaenomelis by using HPLC was established.A Zorbax SB-C18 column was used and the mobile phase was 0.1% oxalic acid.The detector was UV with the flow rate being 1.0mL/min and the detection wave length 261 nm in the HPLC system.The standard working curve of Vitamin C had a good liner relation in the concentration ranging from 20 to 100 mg,the liner equation was A=73.054 2C+50.689 8,and the related coefficient was 0.999 8.The recoveries were 98.81%～102.45% and the relatively standard deviation was equal to 0.861%.This method was simple and fast,and it can exactly detect the content of Vitamin C in Fructus chaenomelis.
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Claims 6
Claim · model-written summary
The vitamin C standard curve was linear from 20 to 100 mg.
Verbatim
e0“The standard working curve of Vitamin C had a good liner relation in the concentration ranging from 20 to 100 mg”
t0“Fructus Chaenomelis”
Claim · model-written summary
The vitamin C calibration equation was A=73.054 2C+50.689 8.
Verbatim
e0“the liner equation was A=73.054 2C+50.689 8”
t0“Fructus Chaenomelis”
Claim · model-written summary
The calibration correlation coefficient was 0.999 8.
Verbatim
e0“the related coefficient was 0.999 8”
t0“Fructus Chaenomelis”
Claim · model-written summary
Recoveries were 98.81%～102.45%.
Verbatim
e0“The recoveries were 98.81%～102.45%”
t0“Fructus Chaenomelis”
Claim · model-written summary
Relative standard deviation was 0.861%.
Verbatim
e0“the relatively standard deviation was equal to 0.861%”
t0“Fructus Chaenomelis”
Claim · model-written summary
The method was judged simple, fast and accurate for vitamin C determination.
Not reportedComparator
Verbatim
e0“This method was simple and fast,and it can exactly detect the content of Vitamin C in Fructus chaenomelis”