Abstract
The tender leafstalks of Ledum palustre var.dilatatum were used as explants for the experiment.Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks,rooting and germplasm conservation in vitro was screened.The results showed that N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration,the frequency of shoots induction was higher than 92.5%;MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting,the rate of rooting was 98%;N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 90-fold proliferation rate was achieved within 30 days.The method of deferring growth with dwarfing was utilized for germplasm conservation in vitro at normal temperature.In vitro culture and germplasm conservation in vitro system of Ledum palustre var.dilatatum was established.
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Claims 5
Claim · model-written summary
Shoot regeneration frequency was higher than 92.5% on N6 with ZT and IAA at the base of tender leafstalks.
- Taxon
- Ledum palustre var.dilatatum
Rhododendron tomentosume5 - Organ / stage
- base of tender leafstalkse6
- Treatment
- N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1e0
- Outcome
- frequency of shoots inductione0
- Direction
- higher thane0
- Value
- 92.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The tender leafstalks of Ledum palustre var.dilatatum were used as explants”
e0“N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration,the frequency of shoots induction was higher than 92.5%”
e6“immediately at base of tender leafstalks”
Claim · model-written summary
Rooting rate was 98% on MS (modified) with IAA and kinetin.
- Taxon
- Ledum palustre var.dilatatumRhododendron tomentosume5
- Treatment
- MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1e1
- Outcome
- rate of rootinge1
- Value
- 98%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The tender leafstalks of Ledum palustre var.dilatatum were used as explants”
e1“MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting,the rate of rooting was 98%”
Claim · model-written summary
Shoots were conserved in vitro for 46 months on N-68 with B9 and phloridzin.
- Taxon
- Ledum palustre var.dilatatumRhododendron tomentosume5
- Treatment
- N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1e2
- Outcome
- germplasm conservation in vitroe2
- Value
- 46 monthse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The tender leafstalks of Ledum palustre var.dilatatum were used as explants”
e2“N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months”
Claim · model-written summary
Stems with one node gave a 90-fold proliferation rate within 30 days.
- Taxon
- Ledum palustre var.dilatatumRhododendron tomentosume5
- Outcome
- proliferation ratee3
- Value
- 90-folde3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The tender leafstalks of Ledum palustre var.dilatatum were used as explants”
e3“a 90-fold proliferation rate was achieved within 30 days”
Claim · model-written summary
Deferring growth with dwarfing allowed in vitro germplasm conservation at normal temperature.
- Taxon
- Ledum palustre var.dilatatumRhododendron tomentosume5
- Treatment
- deferring growth with dwarfinge4
- Outcome
- germplasm conservation in vitroe4
- Setting
- normal temperaturee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The tender leafstalks of Ledum palustre var.dilatatum were used as explants”
e4“The method of deferring growth with dwarfing was utilized for germplasm conservation in vitro at normal temperature”