Abstract
D.villosum is a rich reservoir for genic diversity of High-Molecular-Weight glutenin subunit(HMW-GS).In this paper,one Allelic Specific PCR(AS-PCR)marker for the amplification of the genes coding HMW-GS of D.villosum was developed on the basis of the sequences of 1Va,1Vb,1Vc and common wheat HMW glutenin genes.PCR analysis on HMW glutenin genes of CS and its addition lines showed the specialty of this AS-PCR marker,while analysis on HMW-GS genes of six accessions of D.villosum showed a high degree of length polymorphism,which suggested that the size variation observed in different allelic subunits of D.villosum was mainly due to the different length of the central repetitive domain.Using this AS-PCR marker,seven alleles at Glu-V1 were found among 108 H.villosum individuals from 18 accessions.The frequencies of those alleles were different.The allele b was found in all accessions except W6 7288,and its frequency was 59.55%of 108 individuals,while that of allele d was 25.19%.But allele a as well as f was only found once.Therefore,different genotypes were found in the different individuals from one accession due to the cross-pollination.
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Claims 9
Claim · model-written summary
The AS-PCR marker specifically amplified HMW glutenin genes in CS and addition lines.
Not reportedValue
Verbatim
e0“PCR analysis on HMW glutenin genes of CS and its addition lines showed the specialty of this AS-PCR marker”
e1“HMW-GS of D.villosum”
Claim · model-written summary
HMW-GS genes in six D. villosum accessions showed high length polymorphism.
Verbatim
e0“analysis on HMW-GS genes of six accessions of D.villosum showed a high degree of length polymorphism”
Claim · model-written summary
Variation in allelic subunit size was attributed mainly to central repetitive domain length.
Verbatim
e0“the size variation observed in different allelic subunits of D.villosum was mainly due to the different length of the central repetitive domain”
Claim · model-written summary
Seven Glu-V1 alleles were found in 108 individuals from 18 accessions.
Verbatim
e0“seven alleles at Glu-V1 were found among 108 H.villosum individuals from 18 accessions”
t0“Dasypyum villosum”
Claim · model-written summary
Allele b occurred in all accessions except W6 7288 at a frequency of 59.55%.
Verbatim
e0“The allele b was found in all accessions except W6 7288,and its frequency was 59.55%of 108 individuals”
t0“Dasypyum villosum”
Claim · model-written summary
Allele d frequency was 25.19%.
Verbatim
e0“that of allele d was 25.19%”
t0“Dasypyum villosum”
Claim · model-written summary
Allele a was found once.
Verbatim
e0“allele a as well as f was only found once”
t0“Dasypyum villosum”
Claim · model-written summary
Allele f was found once.
Verbatim
e0“allele a as well as f was only found once”
t0“Dasypyum villosum”
Claim · model-written summary
Different genotypes occurred among individuals within one accession, attributed to cross-pollination.
Verbatim
e0“different genotypes were found in the different individuals from one accession due to the cross-pollination”
t0“Dasypyum villosum”