Abstract
Based on the considerations of possible adverse effects caused by the development of biological technology,biology security has increasingly been the focal issue concerned at home and abroad.In this research,phthalic acid esters(PAEs) were used to treat the seeds of Triticum aestivum so as to study its effects on the seeds germination,including physiological changes and molecular mechanisms,which might undergo the progress of programmed cell death(PCD) after being treated with PAEs.The results showed that the germination rate of the wheat seeds was highly affected by different concentrations of PAEs and with the increase of the concentrations of PAEs,the germination rate showed a decreasing trend.The changes on the activities of reactive oxygen species(ROS),superoxide dismutase(SOD),ascorbate peroxidase(APX) and catalase(CAT) in the ROS pathway of the seeds treated with different concentrations of PAEs at different times,indicated that the activities of SOD and CAT increased at first and then declined,meanwhile,the activity of APX showed a similar trend in the later growth period.The experiment of DNA ladder certified that PAEs at high concentration could cause the Programmed cell death of the seeds.The quantitative expression analysis of the relative genes after PAEs revealed that PAEs could promote the expression of the genes of gr,dhar,vpe and metacaspase,and with theincrease of PAEs,the trends of the relative expression of each gene were basically consistent.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Wheat seed germination decreased as PAEs concentration increased.
- Taxon
- Triticum aestivum
Triticum aestivume1 - Treatment
- PAEse0
- Outcome
- germination ratee0
- Direction
- decreasinge0
- Comparator
- increase of the concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with the increase of the concentrations of PAEs,the germination rate showed a decreasing trend.”
e1“seeds of Triticum aestivum”
Claim · model-written summary
SOD and CAT activity initially increased and then declined after PAEs treatment.
- Taxon
- Triticum aestivumTriticum aestivume2
- Treatment
- PAEse1
- Outcome
- activities of SOD and CATe0
- Direction
- increased at first and then declinede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activities of SOD and CAT increased at first and then declined”
e1“seeds treated with different concentrations of PAEs”
e2“seeds of Triticum aestivum”
Claim · model-written summary
APX activity showed a similar rise then decline in the later growth period.
- Taxon
- Triticum aestivumTriticum aestivume3
- Treatment
- PAEse2
- Outcome
- activity of APXe0
- Direction
- increased at first and then declinede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activity of APX showed a similar trend in the later growth period”
e1“increased at first and then declined”
e2“seeds treated with different concentrations of PAEs”
e3“seeds of Triticum aestivum”
Claim · model-written summary
High concentrations of PAEs induced programmed cell death in seeds.
- Taxon
- Triticum aestivumTriticum aestivume1
- Treatment
- PAEs at high concentratione0
- Outcome
- Programmed cell death of the seedse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PAEs at high concentration could cause the Programmed cell death of the seeds.”
e1“seeds of Triticum aestivum”
Claim · model-written summary
PAEs promoted expression of gr, dhar, vpe and metacaspase, with broadly consistent concentration trends.
- Taxon
- Triticum aestivumTriticum aestivume1
- Treatment
- PAEse0
- Gene
- gr,dhar,vpe and metacaspasee0
- Outcome
- expressione0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PAEs could promote the expression of the genes of gr,dhar,vpe and metacaspase,and with theincrease of PAEs,the trends of the relative expression of each gene were basically consistent.”
e1“seeds of Triticum aestivum”
The same questions in other papers
ester · growth · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.