Abstract
The major factors in the reaction system of ISSR amplification of Torreya jackii,a Class II plant species under state protection in China,was optimized and selected.The optimal ISSR reaction system was determined as follows: 1 ×Taq polymerase corresponding buffer(10mmol/L Tris·HCl pH9.0,50 mmol/L KCl,0.1%Triton X-100),1.5 mmol/L MgCl_2,1U Taq DNA polymerase,10 ng template DNA,6 pmol primer,0.2 mmol/L dATP,dCTP,dGTP,dTTP for each in total 10 μL reaction volume.The optimal annealing temperature was 52.4℃.All the results established a good foundation for the study on the genetic diversity of T.jackii.
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Claims 2
Claim · model-written summary
An optimized ISSR reaction mix was established for Torreya jackii.
- Taxon
- Torreya jackiiTorreya jackiit0
- Outcome
- optimal ISSR reaction systeme0
- Value
- 10 μLe0
- Qualifier
- 1.5 mmol/L MgCl_2,1U Taq DNA polymerase,10 ng template DNA,6 pmol primere0
Verbatim
t0“Torreya jackii”
e0“The optimal ISSR reaction system was determined as follows: 1 ×Taq polymerase corresponding buffer(10mmol/L Tris·HCl pH9.0,50 mmol/L KCl,0.1%Triton X-100),1.5 mmol/L MgCl_2,1U Taq DNA polymerase,10 ng template DNA,6 pmol primer,0.2 mmol/L dATP,dCTP,dGTP,dTTP for each in total 10 μL reaction volume.”
Claim · model-written summary
The optimal annealing temperature was 52.4℃.
- Taxon
- Torreya jackiiTorreya jackiit0
- Outcome
- optimal annealing temperaturee0
- Value
- 52.4℃e0
Verbatim
t0“Torreya jackii”
e0“The optimal annealing temperature was 52.4℃.”