By organism › Green plants › Taxaceae › Torreya jackii

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Most primers also amplified in four other Torreya species.

Taxon: T. fargesii , T . grandis , T. jackii , and T. nuciferaTorreya nuciferaTorreya grandisTorreya fargesiiTorreya jackiie0
Outcome: amplifiede0
Value: Most primerse0

Verbatim

e0
“Most primers also amplified in T. fargesii , T . grandis , T. jackii , and T. nucifera”
✓ located verbatim in the source · abstract, characters 321–407

Title

Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)

Yu Peng Wang; Hai Xiang Cheng; Jian Hui Li; et al. Applications in Plant Sciences 2018. doi:10.1002/aps3.1145

Other claims from this paper (1)

Method / resource Sixteen microsatellite primer sets were identified for Torreya yunnanensis.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

An optimized ISSR reaction mix was established for Torreya jackii.

Taxon: Torreya jackiiTorreya jackiit0
Outcome: optimal ISSR reaction systeme0
Value: 10 μLe0
Qualifier: 1.5 mmol/L MgCl_2,1U Taq DNA polymerase,10 ng template DNA,6 pmol primere0

Verbatim

t0
“Torreya jackii”
✓ located verbatim in the source · title, characters 62–76

e0
“The optimal ISSR reaction system was determined as follows: 1 ×Taq polymerase corresponding buffer(10mmol/L Tris·HCl pH9.0,50 mmol/L KCl,0.1%Triton X-100),1.5 mmol/L MgCl_2,1U Taq DNA polymerase,10 ng template DNA,6 pmol primer,0.2 mmol/L dATP,dCTP,dGTP,dTTP for each in total 10 μL reaction volume.”
✓ located verbatim in the source · abstract, characters 165–464

Title

Establishment of ISSR-PCR Reaction System of Endangered Plant Torreya jackii

Junmin Li Xibei Linxueyuan xuebao 2006