Abstract
Seeds of two hybrid cultivars Zhengdan958 and Xianyu335 were artificially aged by artificial agingchamber. Germination rate, vigor index, germination index were reduced gradually along the seed aging. Seeds oftwo hybrid cultivar germination rate, vigor index and germination index were dropped to the extremely low levelwhen aged eight days. Peroxidase activities, catalase activities of Zhengdan958 and Xianyu335 reduced graduallywhile malonaldehyde content and conductance raised gradually. The 2 750 amplified effective peak value by MSAPbiomarker showed that Zhengdan958 CG methylation level reduced gradually followed seed aging. While the CGmethylation level of Xianyu335 got higher gradually. Seeds genome DNA CHG and CG+CHG methylation levelchanged have no regulation. Zhengdan958 genome DNA CHG and CG+CHG methylation were higher than contrast.Xianyu335 genome DNA CHG methylation was lower than contrast. Zhengdan958 and Xianyu335 DNA occurredmethylation mode variation. Zhengdan958 genome DNA CG hypo mode variation was higher than hyper. WhileXanyu335 DNA CG hyper mode variation was lower than hyper. The correlation analysis of seed vigor index withDNA methylation level showed that seed vigor indexes and Physiological indexes have a higher correlation withDNA CG methylation level.
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Claims 13
Claim · model-written summary
Germination rate, vigor index and germination index declined with seed aging.
- Treatment
- seed aginge0
- Outcome
- Germination rate, vigor index, germination indexe0
- Direction
- reduced graduallye0
Not reportedTaxon
Verbatim
e0“Germination rate, vigor index, germination index were reduced gradually along the seed aging.”
Claim · model-written summary
All three seed vigor metrics reached extremely low levels after eight days of aging.
- Outcome
- germination rate, vigor index and germination indexe0
- Direction
- droppede0
- Qualifier
- aged eight dayse0
Not reportedTaxon
Verbatim
e0“germination rate, vigor index and germination index were dropped to the extremely low levelwhen aged eight days.”
Claim · model-written summary
Peroxidase and catalase activities declined with aging in both cultivars.
- Material
- Zhengdan958 and Xianyu335e0
- Outcome
- Peroxidase activities, catalase activitiese0
- Direction
- reducede0
Not reportedTaxon
Verbatim
e0“Peroxidase activities, catalase activities of Zhengdan958 and Xianyu335 reduced graduallywhile”
Claim · model-written summary
Malonaldehyde and conductance rose with aging.
Not reportedTaxonTreatment
Verbatim
e0“malonaldehyde content and conductance raised gradually.”
Claim · model-written summary
Zhengdan958 CG methylation declined with aging.
Not reportedTaxon
Verbatim
e0“Zhengdan958 CG methylation level reduced gradually followed seed aging.”
Claim · model-written summary
Xianyu335 CG methylation increased with aging.
Not reportedTaxon
Verbatim
e0“CGmethylation level of Xianyu335 got higher gradually.”
Claim · model-written summary
CHG and CG+CHG methylation changes had no regular pattern.
- Outcome
- CHG and CG+CHG methylation levelchanged have no regulatione0
Not reportedTaxon
Verbatim
e0“Seeds genome DNA CHG and CG+CHG methylation levelchanged have no regulation.”
Claim · model-written summary
Zhengdan958 CHG and CG+CHG methylation exceeded the contrast.
Not reportedTaxon
Verbatim
e0“Zhengdan958 genome DNA CHG and CG+CHG methylation were higher than contrast.”
Claim · model-written summary
Xianyu335 CHG methylation was lower than contrast.
Not reportedTaxon
Verbatim
e0“Xianyu335 genome DNA CHG methylation was lower than contrast.”
Claim · model-written summary
Both cultivars showed DNA methylation mode variation.
Not reportedTaxon
Verbatim
e0“Zhengdan958 and Xianyu335 DNA occurredmethylation mode variation.”
Claim · model-written summary
Zhengdan958 CG hypo mode variation exceeded hyper mode.
Not reportedTaxon
Verbatim
e0“Zhengdan958 genome DNA CG hypo mode variation was higher than hyper.”
Claim · model-written summary
Xanyu335 CG hyper mode variation was reported lower than hyper.
Not reportedTaxon
Verbatim
e0“Xanyu335 DNA CG hyper mode variation was lower than hyper.”
Claim · model-written summary
Seed vigor and physiological indexes were highly correlated with DNA CG methylation level.
- Outcome
- seed vigor indexes and Physiological indexese0
- Qualifier
- higher correlation withDNA CG methylation levele0
- Comparator
- DNA CG methylation levele0
Not reportedTaxonGeneDirection
Verbatim
e0“seed vigor indexes and Physiological indexes have a higher correlation withDNA CG methylation level.”