Abstract
Resistance system induced by Fusarium oxysporum f.sp.niveurn in watermelon was described from the whole level,and expression characteristics of the related genes were analyzed.Calhoun Gray,a watermelon variety with high resistance to Fusarium wilt physiological race 1 was used as the test material,and a cDNA library was constructed by suppression subtractive hybridization(SSH) using root tip tissues sampled at 0、6、12、24、36、48 and 72 h after inoculation with race 1 of F.oxysporum f.sp.niveurn or distilled water.The induced clones were verified by reverse Northern dot-blot,and 300 positive clones were randomly sequenced,and the expression of two important genes was studied by RT-PCR.A total of 259 differentially expressed sequences were screened out,and 167 of them were quite similar to the known genes,accounting for 65.5%;Among of them,64 genes are related to disease resistance and defense,accounting for 24.7%.Blast results showed that the resistance related genes of Calhoun Gray included signal transduction,disease defense,transcription factor,secondary metabolism synthesis,plant cell protection,etc.Further analysis indicated that expression of aquaporin and peroxidase genes were both increased after inoculation with Fusarium wilt physiological race 1.The interaction between Calhoun Gray and F.oxysporum involves many aspects,which is similar to that of PI296341.The resistance related genes are mainly those participating in systemic acquired resistance reaction,and genes exclusively expressed in Calhoun Gray but not in PI296341 were gained.These results make a good foundation for further exploration of molecular mechanism between watermelon and F.oxysporum and identification of the key resistant genes.
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Claims 7
Claim · model-written summary
A screen identified 259 differentially expressed sequences.
- Taxon
- watermelon
Citrullus lanatust0 - Outcome
- differentially expressed sequencese0
- Value
- 259e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 259 differentially expressed sequences were screened out”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Of these sequences, 167 resembled known genes, or 65.5%.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- similar to the known genese0
- Value
- 65.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“167 of them were quite similar to the known genes,accounting for 65.5%”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Sixty-four genes, or 24.7%, were related to disease resistance and defense.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- genes are related to disease resistance and defensee0
- Value
- 24.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“64 genes are related to disease resistance and defense,accounting for 24.7%.”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Resistance related genes covered signal transduction, defense, transcription, secondary metabolism and cell protection.
- Taxon
- watermelonCitrullus lanatust0
- Material
- Calhoun Graye0
- Outcome
- resistance related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the resistance related genes of Calhoun Gray included signal transduction,disease defense,transcription factor,secondary metabolism synthesis,plant cell protection,etc.”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Aquaporin gene expression increased after Fusarium inoculation.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inoculation with Fusarium wilt physiological race 1e0
- Gene
- aquaporine0
- Outcome
- expression of aquaporine0
- Direction
- increasede0
FusariumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of aquaporin and peroxidase genes were both increased after inoculation with Fusarium wilt physiological race 1.”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Peroxidase gene expression increased after Fusarium inoculation.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inoculation with Fusarium wilt physiological race 1e0
- Gene
- peroxidasee0
- Outcome
- expression of aquaporin and peroxidase genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of aquaporin and peroxidase genes were both increased after inoculation with Fusarium wilt physiological race 1.”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
Claim · model-written summary
Calhoun Gray had resistance genes expressed exclusively relative to PI296341.
- Taxon
- watermelonCitrullus lanatust0
- Material
- Calhoun Graye0
- Outcome
- genes exclusively expressede0
- Comparator
- PI296341e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes exclusively expressed in Calhoun Gray but not in PI296341 were gained.”
t0“Isolation and expression analysis of resistance related genes in incom patible interaction between watermelon and Fusarium oxysporum f. sp. niveurn”
The same questions in other papers
Fusarium · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
1 in the opposite direction
- negative
inoculation · molecular: expression · Citrullus lanatus (watermelon)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.