Abstract
To investigate the underlying molecular mechanism of watermelon defensin gene,a defensin-like gene ClPDF2.6(GenBank accession No.KC481272) was isolated from watermelon,and its transcription in different organs and under various stresses were analyzed using real-time PCR.The results showed that,ClPDF2.6 contained a 225-bp open reading frame(ORF) encoding 74 amino acids.Alignment of ClPDF2.6 with other plant defensins revealed that ClPDF2.6 had 8 conserved cysteines,which was the potential substrate for plant defensin proteins.Phylogenetic analysis suggested that ClPDF2.6 belonged to the family of Arabidopsis PDF2.Real time PCR analysis revealed that ClPDF2.6 was expressed constitutively in all organs of watermelon,with the highest expression in leaves.The expression of ClPDF2.6 was up-regulated under abscisic acid(SA),methyl jasmonate(JA) and ethephon(ETH) stresses,and was down-regulated in watermelon infected with Fusarium wilt.The results suggested that the signaling molecules played an important role in the activation of ClPDF2.6 gene in watermelon.
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Claims 9
Claim · model-written summary
ClPDF2.6 contains a 225-bp ORF encoding 74 amino acids.
- Taxon
- watermelon
Citrullus lanatust0 - Gene
- ClPDF2.6e0
- Outcome
- open reading frame(ORF)e0
- Value
- 225-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClPDF2.6 contained a 225-bp open reading frame(ORF) encoding 74 amino acids”
t0“watermelon”
Claim · model-written summary
ClPDF2.6 has eight conserved cysteines.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ClPDF2.6e0
- Outcome
- conserved cysteinese0
- Value
- 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClPDF2.6 had 8 conserved cysteines”
t0“watermelon”
Claim · model-written summary
Phylogenetic analysis placed ClPDF2.6 in the Arabidopsis PDF2 family.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ClPDF2.6e0
- Outcome
- family of Arabidopsis PDF2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClPDF2.6 belonged to the family of Arabidopsis PDF2”
t0“watermelon”
Claim · model-written summary
ClPDF2.6 was constitutively expressed in all watermelon organs, highest in leaves.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- leavese0
- Gene
- ClPDF2.6e0
- Outcome
- expressed constitutivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClPDF2.6 was expressed constitutively in all organs of watermelon,with the highest expression in leaves”
Claim · model-written summary
Abscisic acid stress upregulated ClPDF2.6.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- abscisic acid(SA)e0
- Gene
- ClPDF2.6e0
- Outcome
- expression of ClPDF2.6e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClPDF2.6 was up-regulated under abscisic acid(SA),methyl jasmonate(JA) and ethephon(ETH) stresses”
t0“watermelon”
Claim · model-written summary
Methyl jasmonate stress upregulated ClPDF2.6.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- methyl jasmonate(JA)e0
- Gene
- ClPDF2.6e0
- Outcome
- expression of ClPDF2.6e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClPDF2.6 was up-regulated under abscisic acid(SA),methyl jasmonate(JA) and ethephon(ETH) stresses”
t0“watermelon”
Claim · model-written summary
Ethephon stress upregulated ClPDF2.6.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ethephon(ETH) stressese0
- Gene
- ClPDF2.6e0
- Outcome
- expression of ClPDF2.6e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClPDF2.6 was up-regulated under abscisic acid(SA),methyl jasmonate(JA) and ethephon(ETH) stresses”
t0“watermelon”
Claim · model-written summary
Fusarium wilt infection downregulated ClPDF2.6.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- infected with Fusarium wilte0
- Gene
- ClPDF2.6e0
- Outcome
- expression of ClPDF2.6e0
- Direction
- down-regulatede0
FusariumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClPDF2.6 was up-regulated under abscisic acid(SA),methyl jasmonate(JA) and ethephon(ETH) stresses,and was down-regulated in watermelon infected with Fusarium wilt”
Claim · model-written summary
Signaling molecules may activate ClPDF2.6 in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Gene
- ClPDF2.6e0
- Outcome
- activation of ClPDF2.6 genee0
- Qualifier
- signaling moleculese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“signaling molecules played an important role in the activation of ClPDF2.6 gene in watermelon”
The same questions in other papers
Fusarium · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
1 in the opposite direction
- positive
salicylic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →(-)-methyl jasmonate · molecular: expression · Citrullus lanatus (watermelon)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.