Abstract
The objective of this study was to develop an effective protocol to transform glucose oxidase gene (GO gene) into cotton.Studies on several factors affecting cotton transformation,resistant calli induction and screening.Hypocotyls of Gossypium hirsutum L. were used as explants for transformation.Our results indicated that seedings of 5～7d are the most suitable period for transformation and that the seedlings of 3d dark culture plus 2d light culture are more effective. The morphological positons have little different effect on transformation. Among all the factors affecting transformation,virility of Agrobacterium tumefaciens is one of most principal ones and the virility can be measured by culturing hours that are quite conveient and effective. Study also showed that,during the callus induction,levels of medium solidification greatly affect the percentage of callus induction.High level of solidification increases the abnormal calli percent and decreases the normal callus induction percent. The screening times are also discussed.
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Claims 8
Claim · model-written summary
Seedlings aged 5–7 days were most suitable for transformation.
- Taxon
- Gossypium hirsutum L.
Gossypium hirsutumt0 - Organ / stage
- seedings of 5～7de0
- Outcome
- most suitable period for transformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seedings of 5～7d are the most suitable period for transformation”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Three days of dark culture plus two days of light culture improved transformation.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- 3d dark culture plus 2d light culturee0
- Outcome
- more effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seedlings of 3d dark culture plus 2d light culture are more effective”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Morphological position had little effect on transformation.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- morphological positonse0
- Outcome
- little different effect on transformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The morphological positons have little different effect on transformation.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Agrobacterium virility was a principal factor affecting transformation.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0other - Outcome
- most principal onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virility of Agrobacterium tumefaciens is one of most principal ones”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Culture duration was reported to measure Agrobacterium virility effectively.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Outcome
- virilitye0
- Value
- culturing hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the virility can be measured by culturing hours that are quite conveient and effective.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Medium solidification level greatly affected callus induction percentage.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- levels of medium solidificatione0
- Outcome
- percentage of callus inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels of medium solidification greatly affect the percentage of callus induction.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
High solidification increased abnormal callus percentage.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- High level of solidificatione0
- Outcome
- abnormal calli percente0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High level of solidification increases the abnormal calli percent”
t0“Gossypium hirsutum L.”
Claim · model-written summary
High solidification decreased normal callus induction percentage.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- High level of solidificatione0
- Outcome
- normal callus induction percente0
- Direction
- decreasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High level of solidification increases the abnormal calli percent and decreases the normal callus induction percent.”
t0“Gossypium hirsutum L.”