Abstract
At present,scientists are engaged in engineering plants with two or more resistant genes in order to delay and prevent the adaptation in pest species,prolong the usage of life span of transgenic plants and broaden resistant spectrum.Thus,the Bt and Sck(modified CpTI gene)gene,driven by CaMV35S promoter and CLCuV promoter(PRPB),respectively,were transformed into the cotton genome(Sumian 16) by pollen-tube pathway-mediated method in our lab.Five transgenic cotton plants(T0) were obtained by Kanamycin screening.The results of PCR and Southern hybridization indicated that the exogenous genes were stably integrated into cotton genome.Laboratory bioassays showed that the mortality of bollworm larvae(Helicoverpa armigera) accounted for more than 90% at peak flowering and boll developing stage after 5 d fed with new expanded leaves on main stem nodes of the two transgenic plants,312-5 and 332-2,which had higher efficiency against neonate larvae than bivalent transgenic cotton sGK321(unpublished data).The homologous lines(312-5 T2 and 332-2 T2) were obtained in the second generation progeny plants(T2) from the self-fertilized transformed parent tested by Kanamycin resistance and bollworm bioassays.In the present work,these two transgenic homologous lines showed high efficiency to bollworm by insect bioassays.The insect-resistant and susceptible plants were segregated out in F2 populations produced between homologous transgenic lines(312-5 T2,332-2 T2) and three cultivars(Sumian16,R19 and sGK321).The plants with three and four in leaf damage index and 3rd instar larvae survival were classed as the susceptible and those of one and two in leaf damage index and no 3rd instar larvae survival as the resistant after 5d feeding in laboratory bioassays.According to this standard,segregation of resistant and susceptible plants fits 3∶1 ratio in F2 populations crossed among the two homologous transgenic lines and Sumian 16,respectively. This result indicated that the resistance of homologous transgenic lines to Helicoverpa armigera was controlled by one pair of dominant genes.The populations of(312-5T2×sGK321) F1,(332-2T2×sGK321) F1,(312-5T2×R19)F1 and(332-2T2×R19)F1 had the same resistant level to bollworm as their parents.It meant that in heterozygous status,there was no co-suppression phenomenon which caused gene silence and resistance decrease.Additionally,there were 112 vs.12 and 187 vs.20 resistant and susceptible plants segregated out respectively for (312-5T2×sGK321) F2 and(332-2T2×sGK321) F2.These values closely fit 15∶1 segregation ratio.These results showed independent inheritance or different chromosome insertion of Bt genes for transgenic homologous lines and sGK321 strains.Segregation of resistant and susceptible plants also fit 15∶1 ratio in(312-5T2×R19)F2 population,indicating independent inheritance or different chromosome insertion of Bt genes for 312-5T2 and R19.In the population of(332-2T2×R19) F2,all the plants were resistant to bollworm,implying linkage inheritance or the same chromosome insertion of Bt genes for 332-2T2 and R19.So the two homozygous transgenic lines(312-5T2 and 332-2T2) were valuable to develop new transgenic cotton varieties with high efficiency against cotton bollworm.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Five transgenic cotton plants were recovered after kanamycin screening.
- Taxon
- cotton
Gossypium hirsutume0 - Outcome
- Five transgenic cotton plants(T0)e0
- Value
- Fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five transgenic cotton plants(T0) were obtained by Kanamycin screening.”
Claim · model-written summary
PCR and Southern hybridization indicated stable integration of the exogenous genes in cotton.
- Taxon
- cottonGossypium hirsutume0
- Gene
- exogenous genese0
- Outcome
- stably integrated into cotton genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results of PCR and Southern hybridization indicated that the exogenous genes were stably integrated into cotton genome.”
Claim · model-written summary
Bollworm larval mortality exceeded 90% after five days of feeding on leaves of lines 312-5 and 332-2.
- Taxon
- cottonGossypium hirsutumt0
- Material
- 312-5 and 332-2e0
- Organ / stage
- peak flowering and boll developing stagee0
- Interactor
- Helicoverpa armigera
_gusano_cogollero.png?width=60)
Helicoverpa armigerae0pestt1 - Outcome
- mortality of bollworm larvae(Helicoverpa armigera)e0
- Value
- more than 90%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_gusano_cogollero.png?width=500)
Verbatim
e0“the mortality of bollworm larvae(Helicoverpa armigera) accounted for more than 90% at peak flowering and boll developing stage after 5 d fed with new expanded leaves on main stem nodes of the two transgenic plants,312-5 and 332-2”
t0“Cotton”
t1“Helicoverpa armigera”
Claim · model-written summary
The two transgenic lines were more effective against neonate larvae than sGK321.
- Taxon
- cottonGossypium hirsutume0
- Material
- 312-5 and 332-2t1
- Outcome
- efficiency against neonate larvaee0
- Direction
- highere0
- Comparator
- sGK321e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which had higher efficiency against neonate larvae than bivalent transgenic cotton sGK321”
t1“312-5 and 332-2”
Claim · model-written summary
Homozygous lines 312-5 T2 and 332-2 T2 were obtained in the second generation.
- Taxon
- cottonGossypium hirsutumt0
- Material
- 312-5 T2 and 332-2 T2e0
- Outcome
- homologous linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homologous lines(312-5 T2 and 332-2 T2) were obtained in the second generation progeny plants(T2)”
t0“Cotton”
Claim · model-written summary
The two homozygous transgenic lines showed high resistance to bollworm in bioassays.
- Taxon
- CottonGossypium hirsutumt0
- Interactor
- bollworm
.jpg?width=60)
Helicoverpa zeae0pestt1 - Outcome
- high efficiency to bollworme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“these two transgenic homologous lines showed high efficiency to bollworm by insect bioassays”
t0“Cotton”
t1“Helicoverpa armigera”
Claim · model-written summary
Resistance in crosses with Sumian 16 segregated 3:1 and was interpreted as controlled by one dominant gene pair.
- Taxon
- cottonGossypium hirsutumt0
- Material
- Sumian 16e0
- Interactor
- Helicoverpa armigeraHelicoverpa armigerae0pestt1
- Gene
- one pair of dominant genese0
- Outcome
- segregation of resistant and susceptible plantse0
- Value
- 3∶1 ratioe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“segregation of resistant and susceptible plants fits 3∶1 ratio in F2 populations crossed among the two homologous transgenic lines and Sumian 16,respectively. This result indicated that the resistance of homologous transgenic lines to Helicoverpa armigera was controlled by one pair of dominant genes.”
t0“Cotton”
t1“Helicoverpa armigera”
Claim · model-written summary
F1 crosses with sGK321 and R19 retained parental bollworm resistance, suggesting no co-suppression in heterozygotes.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- bollwormHelicoverpa zeae0pestt1
- Outcome
- resistant level to bollworme0
- Direction
- samee0
- Comparator
- their parentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The populations of(312-5T2×sGK321) F1,(332-2T2×sGK321) F1,(312-5T2×R19)F1 and(332-2T2×R19)F1 had the same resistant level to bollworm as their parents.It meant that in heterozygous status,there was no co-suppression phenomenon which caused gene silence and resistance decrease.”
t0“Cotton”
t1“Helicoverpa armigera”
Claim · model-written summary
The 312-5T2 by sGK321 F2 cross yielded 112 resistant and 12 susceptible plants, fitting 15:1 segregation.
- Taxon
- cottonGossypium hirsutumt0
- Material
- (312-5T2×sGK321) F2e0
- Outcome
- resistant and susceptible plantse0
- Value
- 112 vs.12e0
- Qualifier
- 15∶1 segregation ratioe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“112 vs.12 and 187 vs.20 resistant and susceptible plants segregated out respectively for (312-5T2×sGK321) F2 and(332-2T2×sGK321) F2.These values closely fit 15∶1 segregation ratio.”
t0“Cotton”
Claim · model-written summary
The 332-2T2 by sGK321 F2 cross yielded 187 resistant and 20 susceptible plants, fitting 15:1 segregation.
- Taxon
- cottonGossypium hirsutumt0
- Material
- (332-2T2×sGK321) F2e0
- Outcome
- resistant and susceptible plantse0
- Value
- 187 vs.20e0
- Qualifier
- 15∶1 segregation ratioe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“112 vs.12 and 187 vs.20 resistant and susceptible plants segregated out respectively for (312-5T2×sGK321) F2 and(332-2T2×sGK321) F2.These values closely fit 15∶1 segregation ratio.”
t0“Cotton”
Claim · model-written summary
Resistance in the 312-5T2 by R19 F2 cross fit a 15:1 ratio, consistent with independent Bt inheritance.
- Taxon
- cottonGossypium hirsutumt0
- Material
- (312-5T2×R19)F2e0
- Gene
- Bt genese0
- Outcome
- Segregation of resistant and susceptible plantse0
- Value
- 15∶1 ratioe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Segregation of resistant and susceptible plants also fit 15∶1 ratio in(312-5T2×R19)F2 population,indicating independent inheritance or different chromosome insertion of Bt genes for 312-5T2 and R19.”
t0“Cotton”
Claim · model-written summary
All plants in the 332-2T2 by R19 F2 cross resisted bollworm, implying linked Bt inheritance.
- Taxon
- cottonGossypium hirsutumt0
- Material
- (332-2T2×R19) F2e0
- Interactor
- bollwormHelicoverpa zeae0pestt1
- Gene
- Bt genese0
- Outcome
- all the plants were resistant to bollworme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the population of(332-2T2×R19) F2,all the plants were resistant to bollworm,implying linkage inheritance or the same chromosome insertion of Bt genes for 332-2T2 and R19.”
t0“Cotton”
t1“Helicoverpa armigera”
Claim · model-written summary
The authors considered lines 312-5T2 and 332-2T2 valuable for developing bollworm resistant cotton varieties.
- Taxon
- cottonGossypium hirsutume0
- Material
- 312-5T2 and 332-2T2e0
- Interactor
- cotton bollwormHelicoverpa armigerae0pestt1
- Outcome
- develop new transgenic cotton varieties with high efficiency against cotton bollworme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two homozygous transgenic lines(312-5T2 and 332-2T2) were valuable to develop new transgenic cotton varieties with high efficiency against cotton bollworm.”
t1“Helicoverpa armigera”