Claim · model-written summary
Homozygous lines 312-5 T2 and 332-2 T2 were obtained in the second generation.
- Taxon
- cotton
Gossypium hirsutumt0 - Material
- 312-5 T2 and 332-2 T2e0
- Outcome
- homologous linese0
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homologous lines(312-5 T2 and 332-2 T2) were obtained in the second generation progeny plants(T2)”
t0“Cotton”
Title
Tianzhen Zhang Mianhua xuebao 2007
Other claims from this paper (12)
- Observation Five transgenic cotton plants were recovered after kanamycin screening.
- Observation PCR and Southern hybridization indicated stable integration of the exogenous genes in cotton.
- Observation Bollworm larval mortality exceeded 90% after five days of feeding on leaves of lines 312-5 and 332-2.
- Observation The two transgenic lines were more effective against neonate larvae than sGK321.
- Observation The two homozygous transgenic lines showed high resistance to bollworm in bioassays.
- Observation Resistance in crosses with Sumian 16 segregated 3:1 and was interpreted as controlled by one dominant gene pair.
- Observation F1 crosses with sGK321 and R19 retained parental bollworm resistance, suggesting no co-suppression in heterozygotes.
- Observation The 312-5T2 by sGK321 F2 cross yielded 112 resistant and 12 susceptible plants, fitting 15:1 segregation.
- Observation The 332-2T2 by sGK321 F2 cross yielded 187 resistant and 20 susceptible plants, fitting 15:1 segregation.
- Observation Resistance in the 312-5T2 by R19 F2 cross fit a 15:1 ratio, consistent with independent Bt inheritance.
- Observation All plants in the 332-2T2 by R19 F2 cross resisted bollworm, implying linked Bt inheritance.
- Recommendation The authors considered lines 312-5T2 and 332-2T2 valuable for developing bollworm resistant cotton varieties.