Abstract
While the authors have previously developed a method of pistil filament treatment with Agrobacterium cells during blossoming for the transformation of maize generative cells, the mechanism for bacterial T-DNA penetration into the embryo sac remained unknown. This article analyzes the possibility of agrobacterial penetration into the maize embryo via pollen tubes. Microbiological, PCR, and GUS techniques were used to confirm that agrobacteria could spread for up to 20 cm from the sie of inoculation and were detected in maize embryo tissues as aerly as 24 h after inoculation, while they were not revealed after 5-13 days.
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Claims 3
Claim · model-written summary
After pistil filament inoculation, agrobacteria spread up to 20 cm from the inoculation site.
- Organ / stage
- pistil filamentse1
- Interactor
- agrobacteriae0other
- Outcome
- agrobacteria could spreade0
- Value
- up to 20 cme0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“agrobacteria could spread for up to 20 cm from the sie of inoculation”
e1“maize pistil filaments”
Claim · model-written summary
Agrobacteria were detected in maize embryo tissue as early as 24 h after inoculation.
- Organ / stage
- maize embryo tissuese0
- Interactor
- agrobacteriae1other
- Outcome
- detected in maize embryo tissuese0
- Value
- 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were detected in maize embryo tissues as aerly as 24 h after inoculation”
e1“agrobacteria could spread”
t0“maize pistil filaments”
Claim · model-written summary
Agrobacteria were no longer detected 5–13 days after inoculation.
- Organ / stage
- maize embryo tissuese2
- Interactor
- agrobacteriae1other
- Outcome
- not revealede0
- Value
- 5-13 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were not revealed after 5-13 days”
e1“agrobacteria could spread”
e2“maize embryo tissues”
t0“maize pistil filaments”