Abstract
Microtubers of potato cv.‘Favorita'were used as explants in this experiment,the plant expression vectors of sense and antisense PPase gene were driven by the constitutive expression promoter CaMV 35 S,which were constructed and transferred to potato by Agrobacterium-mediated transformation method.And PCR assay confirmed that both sense and antisense PPase gene were integrated into potato genome.Tuber dormancy characteristic of the transgenic plants stored at 4℃and 25℃showed that period of dormancy of the transgenic antisense PPase gene plants were prolonged 2to 3weeks compared to the control,while the dormancy period of the transgenic sense PPase gene plants were shortened 2to 3weeks.The results provide the theoretical evidence for further improving potato tuber dormancy characters using PPase gene.
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Claims 4
Claim · model-written summary
Sense PPase was integrated into the potato genome.
- Taxon
- potato
Solanum tuberosume0 - Gene
- sense PPase genee0
- Outcome
- integrated into potato genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR assay confirmed that both sense and antisense PPase gene were integrated into potato genome”
Claim · model-written summary
Antisense PPase was integrated into the potato genome.
- Taxon
- potatoSolanum tuberosume0
- Gene
- antisense PPase genee0
- Outcome
- integrated into potato genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR assay confirmed that both sense and antisense PPase gene were integrated into potato genome”
Claim · model-written summary
Antisense PPase prolonged potato tuber dormancy by 2 to 3 weeks relative to control.
- Taxon
- potatoSolanum tuberosume1
- Material
- cv.‘Favorita'e1
- Treatment
- transgenic antisense PPase genee0
- Gene
- antisense PPase genee0
- Outcome
- period of dormancye0
- Direction
- prolongede0
- Value
- 2to 3weekse0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“period of dormancy of the transgenic antisense PPase gene plants were prolonged 2to 3weeks compared to the control”
e1“of potato cv.‘Favorita'were used as explants”
Claim · model-written summary
Sense PPase shortened potato tuber dormancy by 2 to 3 weeks.
- Taxon
- potatoSolanum tuberosume1
- Material
- cv.‘Favorita'e1
- Treatment
- transgenic sense PPase genee0
- Gene
- sense PPase genee0
- Outcome
- dormancy periode0
- Direction
- shortenede0
- Value
- 2to 3weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the dormancy period of the transgenic sense PPase gene plants were shortened 2to 3weeks”
e1“of potato cv.‘Favorita'were used as explants”
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