Abstract
The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly overcome by the evolution of the pathogen. In parallel, susceptibility genes (S-genes) are important for pathogenesis, and loss of S-gene function confers increased resistance in several plants, such as rice, wheat, citrus and tomatoes. In this article, we present the mutation and screening of seven putative S-genes in potatoes, including two DMR6 potato homologues. Using a CRISPR/Cas9 system, which conferred co-expression of two guide RNAs, tetra-allelic deletion mutants were generated and resistance against late blight was assayed in the plants. Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight. Plants mutated in StDND1 showed pleiotropic effects, whereas StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies. Additionally, we showed that DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance, but exhibited a growth phenotype, indicating that StDMR6-1 and StDMR6-2 have different functions. To the best of our knowledge, this is the first report on the mutation and screening of putative S-genes in potatoes, including two DMR6 potato homologues.
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Claims 7
Claim · model-written summary
Functional knockouts of StDND1, StCHL1 and StDMR6-1 increased resistance to late blight in potatoes.
- Taxon
- potatoes
Solanum tuberosume1 - Treatment
- Functional knockoutse1
- Gene
- StDND1, StCHL1, and DMG400000582 (StDMR6-1)e1
- Outcome
- resistance against late blighte1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
Claim · model-written summary
Plants with StDND1 mutated showed pleiotropic effects in potato.
- Taxon
- potatoesSolanum tuberosume1
- Gene
- StDND1e2
- Outcome
- pleiotropic effectse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
e2“Plants mutated in StDND1 showed pleiotropic effects”
Claim · model-written summary
StDMR6-1 and StCHL1 mutated potato plants did not exhibit any growth phenotype.
- Taxon
- potatoesSolanum tuberosume1
- Treatment
- mutatede3
- Gene
- StDMR6-1 and StCHL1e3
- Outcome
- growth phenotypee3
- Direction
- did not exhibite3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
e3“StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies”
Claim · model-written summary
StDMR6-1 and StCHL1 mutated plants are good candidates for further agricultural studies in potato.
- Taxon
- potatoesSolanum tuberosume4
- Gene
- StDMR6-1 and StCHL1e3
- Outcome
- good candidates for further agricultural studiese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies”
e4“the mutation and screening of seven putative S-genes in potatoes”
Claim · model-written summary
DMG401026923 (StDMR6-2) knockout mutants did not show increased late blight resistance.
- Taxon
- potatoesSolanum tuberosume1
- Treatment
- knockoute5
- Gene
- DMG401026923 (here denoted StDMR6-2)e5
- Outcome
- late blight resistancee5
- Direction
- did not demonstrate any increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
e5“DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance”
Claim · model-written summary
DMG401026923 (StDMR6-2) knockout mutants exhibited a growth phenotype.
- Taxon
- potatoesSolanum tuberosume1
- Treatment
- knockoute5
- Gene
- DMG401026923 (here denoted StDMR6-2)e5
- Outcome
- growth phenotypee6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
e5“DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance”
e6“but exhibited a growth phenotype”
Claim · model-written summary
StDMR6-1 and StDMR6-2 have different functions in potato.
- Taxon
- potatoesSolanum tuberosume1
- Gene
- StDMR6-1 and StDMR6-2e7
- Outcome
- functionse7
- Direction
- differente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight”
e7“indicating that StDMR6-1 and StDMR6-2 have different functions”
The same questions in other papers
loss of function · growth · Solanum tuberosum (potato)Positive and negative results
8 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.