Abstract
A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bases for biolistic and Agrobacterium-mediated transformation experiments. Friable, nodular and white to pale yellow embryogenic callus cultures (206 mg fresh weight /explant) were obtained from the first basal segments of rice seedlings on Linsmaier and Skoog (LS) medium supplemented with 2,4-D (11.3 microM) and 3.0 microM thiamine-HCL. Plant regeneration was achieved after the transfer of 54 days old embryogenic callus cultures to Murashige and Skoog (MS) medium supplemented with BAP (2.2 microM) and NAA (0.27 microM). In vitro regenerated plants with multiple shoots and roots transferred to sterile soil in growth chamber and maintained in greenhouse exhibited normal growth and were phenotypically similar to plants germinated from seeds.
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Claims 4
Claim · model-written summary
Basal rice leaf segments produced embryogenic callus with 206 mg fresh weight per explant.
- Taxon
- rice
Oryza sativae0 - Material
- Rasie1
- Organ / stage
- first basal segmentse0
- Treatment
- Linsmaier and Skoog (LS) medium supplemented with 2,4-D (11.3 microM) and 3.0 microM thiamine-HCLe0
- Outcome
- embryogenic callus culturese0
- Value
- 206 mg fresh weight /explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Friable, nodular and white to pale yellow embryogenic callus cultures (206 mg fresh weight /explant) were obtained from the first basal segments of rice seedlings on Linsmaier and Skoog (LS) medium supplemented with 2,4-D (11.3 microM) and 3.0 microM thiamine-HCL.”
e1“cultivar Rasi”
Claim · model-written summary
Transfer of embryogenic callus to supplemented MS medium achieved plant regeneration.
- Taxon
- riceOryza sativae1
- Organ / stage
- 54 days old embryogenic callus culturese0
- Treatment
- Murashige and Skoog (MS) medium supplemented with BAP (2.2 microM) and NAA (0.27 microM)e0
- Outcome
- Plant regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration was achieved after the transfer of 54 days old embryogenic callus cultures to Murashige and Skoog (MS) medium supplemented with BAP (2.2 microM) and NAA (0.27 microM).”
e1“indica rice (Oryza sativa)”
Claim · model-written summary
Regenerated rice plants showed normal growth.
- Taxon
- riceOryza sativae1
- Outcome
- normal growthe0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro regenerated plants with multiple shoots and roots transferred to sterile soil in growth chamber and maintained in greenhouse exhibited normal growth and were phenotypically similar to plants germinated from seeds.”
e1“indica rice (Oryza sativa)”
Claim · model-written summary
Regenerated rice plants were phenotypically similar to seed-germinated plants.
- Taxon
- riceOryza sativae1
- Outcome
- phenotypically similare0
- Comparator
- plants germinated from seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro regenerated plants with multiple shoots and roots transferred to sterile soil in growth chamber and maintained in greenhouse exhibited normal growth and were phenotypically similar to plants germinated from seeds.”
e1“indica rice (Oryza sativa)”