Abstract
The present study investigated the effect of different salts on cotton shoot regeneration and transformation efficiency. Two-day-old germinating embryos of a local cotton cultivar (SG-125) were pretreated with 50 mM each of NaCl, CaCl2, and KCl for 60 min. The embryo explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404 harboring a binary plasmid pTF101.1 that carried the insecticidal gene (cry1Ac) under control of wound-inducible promoter (AoPR1) and bilanafos acetyl reductase (bar) gene for plant selection. The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also supplemented with 5 mg/L bialaphos for in vitro screening of the transformed plantlets. The primary transformants were further screened by molecular techniques for integration and expression of the introduced gene. Maximum transformation efficiency (1.10%) was noted on KCl-treated explants compared to nontreated (control) explants. In conclusion, pretreatment of explants with 50 mM KCl for 60 min induced positive effects and triggered shoot regeneration in primary cotton transformants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
Salt-pretreated cotton embryos showed maximum regeneration response on the specified medium.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- salt-pretreatede0
- Outcome
- response on regeneratione0
- Direction
- maximume0
- Qualifier
- MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also supplemented with 5 mg/L bialaphos for in vitro screening of the transformed plantlets.”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
KCl-treated cotton explants achieved maximum transformation efficiency of 1.10% relative to untreated explants.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- KCl-treatede0
- Outcome
- transformation efficiencye0
- Direction
- Maximume0
- Value
- 1.10%e0
- Comparator
- nontreated (control) explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum transformation efficiency (1.10%) was noted on KCl-treated explants compared to nontreated (control) explants.”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Pretreatment with 50 mM KCl for 60 min promoted shoot regeneration in primary cotton transformants.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- pretreatment of explants with 50 mM KCl for 60 mine0
- Outcome
- shoot regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pretreatment of explants with 50 mM KCl for 60 min induced positive effects and triggered shoot regeneration in primary cotton transformants.”
t0“cotton (Gossypium hirsutum L.)”
The same questions in other papers
potassium chloride · growth · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →in vitro · growth · Gossypium hirsutum (cotton)Positive and negative results
29 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- negative
35 papers more in this group
See all 39 papers →salinity · growth · Gossypium hirsutum (cotton)Positive and negative results
15 papers in the same direction as this paper · 91 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
104 papers more in this group
See all 108 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.