Abstract
Direct cryopreservation of overwintering, dormant buds has been applied to nine blackcurrant cultivars, using a 7 day dehydration period at c.-20° C before plunging directly into liquid nitrogen. The buds on shoots thawed from -20° C showed normal development simply by standing them in water and all the cultivars could be successfully recovered (> 58%) by grafting. None of the shoots thawed from liquid nitrogen showed any development after standing in water and all of the grafts failed. Shoots thawed from liquid nitrogen showed significant damage to xylem transport, and the cortical tissues necessary fro successful grafting showed significant loss of membrane semipermeability. However, buds excised from shoots immediately after thawing from liquid nitrogen were viable and could be recovered using in vitro culture. Survival ranged from 88 to 55%, depending upon cultivar.
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Claims 8
Claim · model-written summary
Buds on shoots thawed from -20° C developed normally in water.
Verbatim
e0“The buds on shoots thawed from -20° C showed normal development simply by standing them in water”
t0“blackcurrant”
Claim · model-written summary
All blackcurrant cultivars were recovered by grafting at above 58%.
Verbatim
e0“all the cultivars could be successfully recovered (> 58%) by grafting.”
t0“blackcurrant”
Claim · model-written summary
Shoots thawed from liquid nitrogen did not develop in water.
Verbatim
e0“None of the shoots thawed from liquid nitrogen showed any development after standing in water”
t0“blackcurrant”
Claim · model-written summary
All grafts from liquid nitrogen thawed shoots failed.
Verbatim
e0“all of the grafts failed.”
t0“blackcurrant”
Claim · model-written summary
Liquid nitrogen thawed shoots had significant xylem transport damage.
Verbatim
e0“Shoots thawed from liquid nitrogen showed significant damage to xylem transport”
t0“blackcurrant”
Claim · model-written summary
Cortical tissues needed for grafting significantly lost membrane semipermeability.
Verbatim
e0“the cortical tissues necessary fro successful grafting showed significant loss of membrane semipermeability.”
t0“blackcurrant”
Claim · model-written summary
Buds excised immediately after liquid nitrogen thawing remained viable and recoverable by in vitro culture.
Not reportedComparator
Verbatim
e0“buds excised from shoots immediately after thawing from liquid nitrogen were viable and could be recovered using in vitro culture.”
t0“blackcurrant”
Claim · model-written summary
Survival after in vitro recovery ranged from 88 to 55% by cultivar.
Verbatim
e0“Survival ranged from 88 to 55%, depending upon cultivar.”
t0“blackcurrant”