Abstract
Introduction: UPLC based metabolite profiling was employed to evaluate the chemical constituents of Memecylon talbotianum Brandis extract and its antibacterial activity was studied in vitro. Methods: Methanol extracts of M. talbotianum was subjected to UPLC-PDA-ESI/HDMS metabolite profiling. The antibacterial activity was determined against human pathogens through disc diffusion, Minimum inhibitory concentration (MIC), Minimum biofilm inhibitory concentration (MBIC) test as visualized by Alamar blue and confocal laser scanning microscopy. Results: UPLC-PDA-ESI/HDMS analysis identified eighteen metabolites, synapoyl-hexose-formic acid, kaempferol 3-O-feruloylhexosyl rhamnoside, 6-C-arabinosyl-8-C-glucosylapigenin and isorhamnetin-3-O-glycoside-7-O-glycoside as the main constituents for the first time from this plant. A broad spectrum of antibacterial activity against to test human pathogens (MIC=54 mg/ mL; Gram-positive bacteria) causing lysis at 24 h incubation was reported, resulting in nearly a 4 log10 CFU / mL drop in cell viability at 1.6 X MIC (Gram-positive) for this extract. The extract at 2 fold MIC inhibited the bacterial biofilm formation and at 8 x MIC eradicated biofilms. However, a higher concentration of this extract was identified in this study for a similar effect on Gram-negative bacteria. Conclusion: The presence of these compounds could contribute to their in vitro inhibitory activities against pathogenic bacterial strains indicating its potential as medicinal agents in treatment and prevention of diseases of bacterial origin.
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Claims 9
Claim · model-written summary
UPLC-PDA-ESI/HDMS profiling identified eighteen metabolites in Memecylon talbotianum.
- Taxon
- Memecylon talbotianum Brandis
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Memecylon talbotianumt0 - Outcome
- metabolitese1
- Value
- eighteen metabolitese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Memecylon talbotianum Brandis”
e1“UPLC-PDA-ESI/HDMS analysis identified eighteen metabolites”
Claim · model-written summary
Synapoyl-hexose-formic acid, kaempferol 3-O-feruloylhexosyl rhamnoside and 6-C-arabinosyl-8-C-glucosylapigenin were main constituents of Memecylon talbotianum.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Outcome
- synapoyl-hexose-formic acid, kaempferol 3-O-feruloylhexosyl rhamnoside, 6-C-arabinosyl-8-C-glucosylapigenine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e2“synapoyl-hexose-formic acid, kaempferol 3-O-feruloylhexosyl rhamnoside, 6-C-arabinosyl-8-C-glucosylapigenin”
Claim · model-written summary
Isorhamnetin-3-O-glycoside-7-O-glycoside was a main constituent of Memecylon talbotianum.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Outcome
- isorhamnetin-3-O-glycoside-7-O-glycosidee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e3“isorhamnetin-3-O-glycoside-7-O-glycoside as the main constituents for the first time from this plant”
Claim · model-written summary
The Memecylon talbotianum extract showed a broad spectrum of antibacterial activity against test human pathogens (MIC=54 mg/ mL), with Gram-positive bacteria affected.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Interactor
- human pathogense4pathogen
- Outcome
- antibacterial activitye4
- Value
- MIC=54 mg/ mLe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e4“A broad spectrum of antibacterial activity against to test human pathogens (MIC=54 mg/ mL; Gram-positive bacteria) causing lysis at 24 h incubation was reported”
Claim · model-written summary
At 1.6 X MIC the extract dropped cell viability of Gram-positive bacteria by nearly 4 log10 CFU/mL.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Treatment
- 1.6 X MICe5
- Interactor
- Gram-positive bacteriae4pathogen
- Outcome
- cell viabilitye5
- Direction
- drope5
- Value
- nearly a 4 log10 CFU / mLe5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e4“A broad spectrum of antibacterial activity against to test human pathogens (MIC=54 mg/ mL; Gram-positive bacteria) causing lysis at 24 h incubation was reported”
e5“resulting in nearly a 4 log10 CFU / mL drop in cell viability at 1.6 X MIC (Gram-positive) for this extract”
Claim · model-written summary
At 2 fold MIC the extract inhibited bacterial biofilm formation.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Treatment
- 2 fold MICe6
- Outcome
- bacterial biofilm formatione6
- Direction
- inhibitede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e6“The extract at 2 fold MIC inhibited the bacterial biofilm formation”
Claim · model-written summary
At 8 x MIC the extract eradicated biofilms.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Treatment
- 8 x MICe7
- Outcome
- biofilmse7
- Direction
- eradicatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e7“at 8 x MIC eradicated biofilms”
Claim · model-written summary
A higher concentration of the extract was needed for a similar effect on Gram-negative bacteria.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Treatment
- a higher concentration of this extracte1
- Interactor
- Gram-negative bacteriae1pathogen
- Outcome
- a similar effecte1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e1“However, a higher concentration of this extract was identified in this study for a similar effect on Gram-negative bacteria”
Claim · model-written summary
The compounds of Memecylon talbotianum could contribute to its in vitro inhibitory activity against pathogenic bacterial strains.
- Taxon
- Memecylon talbotianum BrandisMemecylon talbotianumt0
- Interactor
- pathogenic bacterial strainse1pathogen
- Compound
- these compoundse1
- Outcome
- in vitro inhibitory activitiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Memecylon talbotianum Brandis”
e1“The presence of these compounds could contribute to their in vitro inhibitory activities against pathogenic bacterial strains”