Abstract
Due to apparent participation of plant lipoxygenases (LOXs) in the biosynthetic pathways for jasmonic acid, methyl jasmonate, traumatin, and several C-6 volatile compounds, LOXs are believed to have a role in senescence, plant growth and development, and wound- and pathogen-induced defense responses. Multiple functions that are ascribed to this enzyme family are in accordance with the heterogeneity of LOX isozyme forms. It is possible that different LOX isoforms may be involved in different physiological processes. In our search for a gene that encodes a LOX isozyme form specifically involved in potato defense responses against pests and pathogens, we have screened an abscisic acid-induced potato leaf cDNA library, and we have isolated, sequenced, and characterized a cDNA clone that we have designated POTLX-3. The high sequence homology of our cDNA clone to other reported plant LOX genes provided evidence that POTLX-3 is a lipoxygenase. This cDNA clone represents a novel potato LOX gene in that it shares the least nucleotide and amino acid sequence homology to other isolated potato LOX genes. Northern analysis indicated that POTLX-3 transcripts did not accumulate in untreated potato leaves, but it was highly induced by treatment with physiological levels of ethylene. Northern analysis also was performed to study whether the POTLX-3 mRNA accumulation could be induced by other plant hormones that affect expression of the other plant LOX and defense-related genes. Treatment of potato leaves with methyl jasmonate, abscisic acid, gibberellic acid, auxin (NAA), and cytokinin (BA) did not induce POTLX-3 gene expression. Because the pattern of POTLX-3 gene expression is similar to that of pathogenesis-related (PR) proteins, especially the PR-1 and PR-5 groups, we suspect that POTLX-3 may be involved specifically in ethylene-induced defense responses against pathogens.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The authors isolated and characterized a potato leaf cDNA clone named POTLX-3.
- Taxon
- potato
Solanum tuberosume0 - Gene
- POTLX-3e0
- Outcome
- cDNA clonee0
- Value
- POTLX-3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato leaf cDNA library, and we have isolated, sequenced, and characterized a cDNA clone that we have designated POTLX-3”
Claim · model-written summary
Sequence homology supported identification of POTLX-3 as a lipoxygenase.
- Taxon
- potatoSolanum tuberosume1
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 is a lipoxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high sequence homology of our cDNA clone to other reported plant LOX genes provided evidence that POTLX-3 is a lipoxygenase”
e1“potato LOX genes”
Claim · model-written summary
POTLX-3 had the least sequence homology to other isolated potato LOX genes.
- Taxon
- potatoSolanum tuberosume0
- Gene
- POTLX-3e2
- Outcome
- nucleotide and amino acid sequence homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it shares the least nucleotide and amino acid sequence homology to other isolated potato LOX genes”
e1“This cDNA clone represents a novel potato LOX gene”
e2“POTLX-3 transcripts did not accumulate”
Claim · model-written summary
POTLX-3 transcripts did not accumulate in untreated potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- leavese0
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 transcripts did not accumulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“POTLX-3 transcripts did not accumulate in untreated potato leaves”
Claim · model-written summary
Ethylene strongly induced POTLX-3 transcript accumulation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- physiological levels of ethylenee0
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 transcriptse0
- Direction
- highly inducede0
- Comparator
- untreated potato leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“POTLX-3 transcripts did not accumulate in untreated potato leaves, but it was highly induced by treatment with physiological levels of ethylene”
Claim · model-written summary
Methyl jasmonate, abscisic acid, and gibberellic acid did not induce POTLX-3 expression.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- methyl jasmonate, abscisic acid, gibberellic acide0
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 gene expressione0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of potato leaves with methyl jasmonate, abscisic acid, gibberellic acid, auxin (NAA), and cytokinin (BA) did not induce POTLX-3 gene expression”
Claim · model-written summary
Auxin did not induce POTLX-3 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- auxin (NAA)e0
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 gene expressione0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin (NAA), and cytokinin (BA) did not induce POTLX-3 gene expression”
e1“Treatment of potato leaves with methyl jasmonate”
Claim · model-written summary
Cytokinin did not induce POTLX-3 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- cytokinin (BA)e0
- Gene
- POTLX-3e0
- Outcome
- POTLX-3 gene expressione0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytokinin (BA) did not induce POTLX-3 gene expression”
e1“Treatment of potato leaves with methyl jasmonate”
Claim · model-written summary
The authors suspect POTLX-3 participates in ethylene-induced pathogen defense.
- Taxon
- potatoSolanum tuberosume1
- Gene
- POTLX-3e0
- Outcome
- ethylene-induced defense responses against pathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we suspect that POTLX-3 may be involved specifically in ethylene-induced defense responses against pathogens”
e1“potato defense responses against pests and pathogens”
The same questions in other papers
Gibberellic acid · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
N-benzyladenine · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →(-)-methyl jasmonate · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →1-naphthaleneacetic acid · molecular: expression · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- no effect
class · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.