Abstract
EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions.
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Claims 5
Claim · model-written summary
Seven days of glyphosate treatment reduced barley EPSPS activity to 80% of untreated seedlings.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- glyphosate treatmente0
- Gene
- EPSPSe0
- Outcome
- EPSPS activitye0
- Direction
- reducede0
- Value
- 80%e0
- Comparator
- untreated seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“barley”
e0“After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings”
Claim · model-written summary
At 1 mM PEP, 2 μM glyphosate inhibited EPSPS by 18%.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Glyphosate at 2 μM concentratione0
- Gene
- EPSPSe0
- Outcome
- EPSPSe0
- Direction
- inhibitede0
- Value
- 18%e0
- Qualifier
- PEP concentration of 1 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley”
e0“Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM”
Claim · model-written summary
At 0.1 mM PEP, 2 μM glyphosate inhibition increased to 64%.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Glyphosate at 2 μM concentratione0
- Gene
- EPSPSe0
- Outcome
- inhibitione0
- Direction
- increasede0
- Value
- 64%e0
- Qualifier
- PEP concentration was 0.1 mMe0
- Comparator
- PEP concentration of 1 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley”
e0“Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM”
Claim · model-written summary
At 0.1 mM PEP, 1.5 μM glyphosate reduced EPSPS activity by 50%.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- 1.5 μM glyphosate concentratione0
- Gene
- EPSPSe0
- Outcome
- EPSPS activitye0
- Direction
- reducede0
- Value
- 50%e0
- Qualifier
- 0.1 mM of PEPe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley”
e0“1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP”
Claim · model-written summary
At 1 mM PEP, 0.1 mM glyphosate was needed for the reported EPSPS reduction.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- 0.1 mM of glyphosatee0
- Gene
- EPSPSe0
- Outcome
- EPSPS activitye0
- Direction
- reducede0
- Value
- 50%e0
- Qualifier
- 1 mM was present in the reactionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley”
e0“1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions”
The same questions in other papers
glyphosate · disease · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.