Abstract
Buds from rhizomes of Curcuma longa L. variety 'Chumphon' and Curcuma mangga Valeton & Zijp variety 'Phetchaburi' were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L N6-benzyladenine (BA) for multiple shoot induction. Their shoots were cultured on MS medium supplemented with various concentrations of one of two plant growth regulators or a combination of both—2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA). Interestingly, the medium containing both auxins (5 mg/L 2,4-D and 5 mg/L NAA) was best for somatic embryo induction after culturing for 4 weeks. Somatic embryo formation reached 87.50% for Curcuma longa and 95.83% for Curcuma mangga with a high quality of loose, friable and yellowish characters. The best conditions for the formation of shootlets occurred after transferring the somatic embryo to MS medium supplemented with 3.0 mg/L BA, 0.5 mg/L NAA and 3% maltose. The shootlets were rooted by transferring to MS medium containing 3.0 mg/L NAA. This is the first report of a complete in vitro regeneration system from somatic embryos of C. longa and C. mangga which was further used for gene manipulation in these plants. Diketide CoA synthase (DCS) and curcumin synthase (CURS) genes, which are the two genes involved in curcuminoid biosynthesis in turmeric, were cloned and transferred to these two species using Agrobacterium-mediated transformation. The presence of both target and marker genes, hpt, in the transformed somatic embryos was confirmed by polymerase chain reaction assay. After culturing, the transformed somatic embryos could survive for 4 weeks.
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Claims 8
Claim · model-written summary
A medium with both auxins was best for somatic embryo induction after four weeks.
- Taxon
- Curcuma longa
_W_IMG_2440.jpg?width=60)
Curcuma longae1 - Treatment
- 5 mg/L 2,4-D and 5 mg/L NAAe0
- Outcome
- somatic embryo inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
e0“the medium containing both auxins (5 mg/L 2,4-D and 5 mg/L NAA) was best for somatic embryo induction after culturing for 4 weeks.”
e1“Curcuma longa L. variety”
Claim · model-written summary
Somatic embryo formation reached 87.50% in Curcuma longa.
- Taxon
- Curcuma longaCurcuma longae0
- Outcome
- Somatic embryo formatione0
- Value
- 87.50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryo formation reached 87.50% for Curcuma longa and 95.83% for Curcuma mangga”
Claim · model-written summary
Somatic embryo formation reached 95.83% in Curcuma mangga.
- Taxon
- Curcuma manggaCurcuma manggae0
- Outcome
- Somatic embryo formatione0
- Value
- 95.83%e0
Verbatim
e0“Somatic embryo formation reached 87.50% for Curcuma longa and 95.83% for Curcuma mangga”
Claim · model-written summary
BA, NAA, and maltose medium gave the best shootlet formation conditions in Curcuma.
- Taxon
- Curcuma longaCurcuma longae1
- Treatment
- 3.0 mg/L BA, 0.5 mg/L NAA and 3% maltosee0
- Outcome
- formation of shootletse0
- Direction
- beste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best conditions for the formation of shootlets occurred after transferring the somatic embryo to MS medium supplemented with 3.0 mg/L BA, 0.5 mg/L NAA and 3% maltose.”
e1“Curcuma longa L. variety”
Claim · model-written summary
Shootlets rooted on medium with 3.0 mg/L NAA.
- Taxon
- Curcuma longaCurcuma longae1
- Treatment
- 3.0 mg/L NAAe0
- Outcome
- shootlets were rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shootlets were rooted by transferring to MS medium containing 3.0 mg/L NAA.”
e1“Curcuma longa L. variety”
Claim · model-written summary
DCS and CURS genes were cloned and transferred into both Curcuma species.
- Taxon
- turmericCurcuma longae0
- Gene
- Diketide CoA synthase (DCS) and curcumin synthase (CURS)e0
- Outcome
- were cloned and transferrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diketide CoA synthase (DCS) and curcumin synthase (CURS) genes, which are the two genes involved in curcuminoid biosynthesis in turmeric, were cloned and transferred to these two species using Agrobacterium-mediated transformation.”
Claim · model-written summary
PCR confirmed target and marker genes in transformed somatic embryos.
- Taxon
- Curcuma longaCurcuma longae1
- Gene
- hpte0
- Outcome
- presence of both target and marker genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of both target and marker genes, hpt, in the transformed somatic embryos was confirmed by polymerase chain reaction assay.”
e1“Curcuma longa L. variety”
Claim · model-written summary
Transformed somatic embryos survived four weeks of culture.
- Taxon
- Curcuma longaCurcuma longae1
- Outcome
- transformed somatic embryos could survivee0
- Value
- 4 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After culturing, the transformed somatic embryos could survive for 4 weeks.”
e1“Curcuma longa L. variety”
The same questions in other papers
2,4-D · growth · Curcuma longa (turmeric)Results agree
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- positive
- positive
1-naphthaleneacetic acid · growth · Curcuma longa (turmeric)Results agree
9 papers in the same direction as this paper
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.