Abstract
The present experiment was designed to assess the effects of seed soaking with 24-epibrassinolide (EBR) on the physiology of Brassica juncea L. seedlings grown under imidacloprid (IMI) toxicity. Application of EBR increased the length of seedlings, dry weight, and pigment contents, polyphenols, total phenols and organic acids under IMI toxicity. The expression of genes coding key enzymes of pigment, phenols, polyphenols and organic acid biosynthetic pathways was also studied including CHLASE (chlorophyllase), PSY (phytoene synthase), CHS (chalcone synthase) and PAL (phenylalanine ammonialyase), CS (citrate synthase), SUCLG1 (succinyl Co-A ligase,), SDH (succinate dehydrogenase), FH (fumarate hydratase), MS (malate synthase). Multiple linear regression analysis revealed that IMI application regressed negatively on seedling length, dry weight and total chlorophyll content. However, EBR seed treatment regressed positively on all of the parameters studied. Moreover, interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate, and expression of PSY and PAL. Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression of CHS and all genes studied related to organic acid metabolism. In conclusion, EBR enhanced the growth and contents of all studied metabolites by regulating the gene expression of B. juncea seedlings under IMI stress.
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Claims 10
Claim · model-written summary
EBR raised seedling length and dry weight under IMI toxicity.
- Taxon
- Brassica juncea L.
Brassica junceat0 - Treatment
- EBRe0
- Outcome
- length of seedlings, dry weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of EBR increased the length of seedlings, dry weight”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
EBR raised pigment, polyphenol, phenol and organic acid contents under IMI toxicity.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- EBRe0
- Outcome
- pigment contents, polyphenols, total phenols and organic acidse0
- Direction
- increasede0
- Qualifier
- under IMI toxicitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of EBR increased the length of seedlings, dry weight, and pigment contents, polyphenols, total phenols and organic acids under IMI toxicity”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
IMI was negatively related to seedling length, dry weight and chlorophyll.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- IMI applicatione0
- Outcome
- seedling length, dry weight and total chlorophyll contente0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IMI application regressed negatively on seedling length, dry weight and total chlorophyll content”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
EBR treatment showed positive regressions for all studied parameters.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- EBR seed treatmente0
- Outcome
- all of the parameters studiede0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EBR seed treatment regressed positively on all of the parameters studied”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
IMI by EBR interaction had positive regression for growth parameters.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- interaction between IMI and EBRe0
- Outcome
- growth parameterse0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction between IMI and EBR showed positive regression for growth parameters”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
IMI by EBR interaction had positive regression for pigments, polyphenols, phenol and malate.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- interaction between IMI and EBRe0
- Outcome
- content of pigments, total polyphenol, total phenol and malatee0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
IMI by EBR interaction was positively related to PSY and PAL expression.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- interaction between IMI and EBRe0
- Gene
- PSY and PALe0
- Outcome
- expression of PSY and PALe0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate, and expression of PSY and PAL”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
Claim · model-written summary
IMI by EBR interaction was negative for fumarate, succinate and citrate contents.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- IMI and EBRe1
- Outcome
- contents of fumarate, succinate and citratee0
- Direction
- Negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Negative interactions were noticed for the contents of fumarate, succinate and citrate”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
e1“interaction between IMI and EBR showed positive regression for growth parameters”
Claim · model-written summary
IMI by EBR interaction was negative for CHS expression.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- IMI and EBRe1
- Gene
- CHSe0
- Outcome
- expression of CHSe0
- Direction
- Negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression of CHS”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
e1“interaction between IMI and EBR showed positive regression for growth parameters”
Claim · model-written summary
IMI by EBR interaction was negative for organic acid metabolism gene expression.
- Taxon
- Brassica juncea L.Brassica junceat0
- Treatment
- IMI and EBRe1
- Outcome
- expression of CHS and all genes studied related to organic acid metabolisme0
- Direction
- Negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression of CHS and all genes studied related to organic acid metabolism”
t0“Brassica juncea L.”
t1“Brassica juncea L.”
e1“interaction between IMI and EBR showed positive regression for growth parameters”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.