Influence of Drought Stress, Vermicompost and N Fertilizer on Safflower Leaves Antioxidant Enzymes Activity

Abstract

Superoxide dismutase (SOD), Peroxidase activity (POD), catalase (CAT) and glutathione peroxidase (GPX) are antioxidant enzymes which have important role in the metabolic reactive oxygen species (ROS) and defense against oxidative stress damage.Antioxidant enzymes activity increases in plant cells as a response to environmental stresses.The objective of this study was to evaluate the effects of vermicompost and N fertilizer application on the antioxidant enzyme metabolism (CAT and POD) in sunflower under drought stress. To investigate the effect of vermicompost and N fertilizer application on antioxidant enzymes activity under water deficit stress of Safflower in 2012 at Khorramabad-Lorestan , Iran. The experiment was laid out Split plotfactorial in Randomized Complete Block Design with 3 replications. Treatments were, Vermicompost rate in 4 levels (V1=0 (control), V2=2, V3=4 and V4=6 ton Vermicompost ha -1 ) and Nitrogen rate, (N1= 0 (control), N2= 84 (30% less from N3), N3= 120 (from the soil analyze lab), N4= 154 (30% more than N3) kg N ha -1 ), and Irrigation as the main factor in two levels, S1= Control and S2=Water Stress (stress from bloom growth stages). And N fertilizer was in the the form of Urea. Results showed that the activity of these enzymes was significantly different (a= 1%) between control and water stress treatments. The water stress increased antioxidant enzymes activities and whit application Vermicompost and Nitrogen the antioxidant enzymes activity decreased then the control treatments.

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