Abstract
Lepidium draba through the production of anti-cancer and anti-microbial compound, sulforaphane, is considered a valuable medicinal plants.Therefore, optimization of in vitro tissue culture to facilitate genetic transformation and improvement of traits in this plant is important. This study was conducted, by cotyledon, stem and root explants in MS medium in the form of two separate tests. Data were analyzed in a factorial experiment based on completely randomized design. In the first experiment with different concentrations of growth regulators NAA and BAP, the highest response rate, direct shoot regeneration rate and direct shoot generation were related to root explants. The highest root production rate and root generation were showed in cotyledons explants. 1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants. Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation. Callus induction rate and callus initiation of stem explants were more than others, but the callus regeneration rate in three studied explants showed no significant difference. The highest shoot generation from regenerated callus was observed in the cotyledons explants. The second experiment with a combination of different concentrations of 2,4-D and BAP, under the influence of two treatments darkness and 16h photoperiod was performed. The highest investigated traits were in the 16h photoperiod. Cotyledons and stem explants were showed the highest callus induction rate and leaf explants showed the greatest weight of callus; While the highest callus regeneration rate and callus growth rate were obtained from the root explants. From leaf explants, the highest callus weight was obtained from 0.5 mgl-1 2,4-D with 3 mgl-1 BAP and the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP. Also the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants.
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Claims 17
Claim · model-written summary
the highest response rate, direct shoot regeneration rate and direct shoot generation were related to root explants
- Taxon
- Lepidium draba
Lepidium drabat0 - Organ / stage
- root explantse0
- Outcome
- response rate, direct shoot regeneration rate and direct shoot generatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest response rate, direct shoot regeneration rate and direct shoot generation were related to root explants”
t0“Lepidium draba L.”
Claim · model-written summary
The highest root production rate and root generation were showed in cotyledons explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- cotyledons explantse0
- Outcome
- root production rate and root generatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest root production rate and root generation were showed in cotyledons explants”
t0“Lepidium draba L.”
Claim · model-written summary
1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- root explantse0
- Treatment
- 1 mgl-1 BAP without NAAe0
- Outcome
- direct shoot generatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants”
t0“Lepidium draba L.”
Claim · model-written summary
Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- Cotyledons explantse0
- Treatment
- 2 mgl-1 NAA and 0.5 mgl-1 BAPe0
- Outcome
- Root production rate and the highest root generatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation”
t0“Lepidium draba L.”
Claim · model-written summary
Callus induction rate and callus initiation of stem explants were more than others
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- stem explantse0
- Outcome
- Callus induction rate and callus initiatione0
- Direction
- moree0
- Comparator
- otherse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction rate and callus initiation of stem explants were more than others”
t0“Lepidium draba L.”
Claim · model-written summary
the callus regeneration rate in three studied explants showed no significant difference
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- three studied explantse0
- Outcome
- callus regeneration ratee0
- Direction
- no significant differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the callus regeneration rate in three studied explants showed no significant difference”
t0“Lepidium draba L.”
Claim · model-written summary
The highest shoot generation from regenerated callus was observed in the cotyledons explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- cotyledons explantse0
- Outcome
- shoot generation from regenerated calluse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot generation from regenerated callus was observed in the cotyledons explants”
t0“Lepidium draba L.”
Claim · model-written summary
The highest investigated traits were in the 16h photoperiod
- Taxon
- Lepidium drabaLepidium drabat0
- Treatment
- 16h photoperiode0
- Outcome
- investigated traitse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest investigated traits were in the 16h photoperiod”
t0“Lepidium draba L.”
Claim · model-written summary
Cotyledons and stem explants were showed the highest callus induction rate
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- Cotyledonse0
- Outcome
- callus induction ratee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledons and stem explants were showed the highest callus induction rate”
t0“Lepidium draba L.”
Claim · model-written summary
Cotyledons and stem explants were showed the highest callus induction rate
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- stem explantse0
- Outcome
- callus induction ratee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledons and stem explants were showed the highest callus induction rate”
t0“Lepidium draba L.”
Claim · model-written summary
leaf explants showed the greatest weight of callus
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- leaf explantse0
- Outcome
- weight of calluse0
- Direction
- greateste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaf explants showed the greatest weight of callus”
t0“Lepidium draba L.”
Claim · model-written summary
the highest callus regeneration rate and callus growth rate were obtained from the root explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- root explantse0
- Outcome
- callus regeneration rate and callus growth ratee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest callus regeneration rate and callus growth rate were obtained from the root explants”
t0“Lepidium draba L.”
Claim · model-written summary
From leaf explants, the highest callus weight was obtained from 0.5 mgl-1 2,4-D with 3 mgl-1 BAP
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- leaf explantse0
- Treatment
- 0.5 mgl-1 2,4-D with 3 mgl-1 BAPe0
- Outcome
- callus weighte0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From leaf explants, the highest callus weight was obtained from 0.5 mgl-1 2,4-D with 3 mgl-1 BAP”
t0“Lepidium draba L.”
Claim · model-written summary
the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- leaf explantse1
- Treatment
- 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAPe0
- Outcome
- growth rate of calluse0
- Direction
- moste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP”
e1“From leaf explants”
t0“Lepidium draba L.”
Claim · model-written summary
the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- leaf explantse1
- Treatment
- 1 mgl-1 2,4-D and 3 mgl-1 BAPe0
- Outcome
- growth rate of calluse0
- Direction
- moste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP”
e1“From leaf explants”
t0“Lepidium draba L.”
Claim · model-written summary
the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- root explantse0
- Treatment
- 2 mgl-1 2,4-D with 2 mgl-1 BAPe0
- Outcome
- callus regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants”
t0“Lepidium draba L.”
Claim · model-written summary
the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants
- Taxon
- Lepidium drabaLepidium drabat0
- Organ / stage
- root explantse0
- Treatment
- 3 mgl-1 BAPe1
- Outcome
- callus regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants”
e1“2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP”
t0“Lepidium draba L.”