Abstract
DNA was extracted from leaves of various Malus genotypes and used to screen synthetic decamer oligonucleotide primers. Samples from the following two groups were bulked: 1) seven scab-susceptible apple cultivars, and 2) 15 scab-resistant apple genotypes derived by introgressive hybridization from the previous group of cultivars. A third sample consisted of DNA extracted from Malus floribunda Sieb. clone 821, the original source of apple scab resistance for all genotypes in the second group. A total of 59 primers from kits A, L, and R (Operon Technologies) were screened. Amplified fragments were obtained for 93% of the primers tested, while random amplified polymorphic DNA (RAPD) fragments were detected among samples for 76% of the primers. One primer, A15, amplified a unique band in both M. floribunda clone 821 and the bulked scab-resistant sample. This RAPD marker, designated OA15 900 , identifies an amplified, introgressed fragment that likely corresponds to a region of the genome that may serve as a modifier for the scab resistance gene block V, derived from M. floribunda clone 821.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Amplified fragments were obtained with 93% of screened primers.
Verbatim
e0“Amplified fragments were obtained for 93% of the primers tested”
Claim · model-written summary
RAPD fragments varied among samples for 76% of screened primers.
Verbatim
e0“random amplified polymorphic DNA (RAPD) fragments were detected among samples for 76% of the primers”
Claim · model-written summary
Primer A15 amplified a unique band in M. floribunda clone 821 and the resistant bulk.
- Taxon
- M. floribunda
.jpg?width=60)
Malus floribundae0 - Material
- clone 821e0
- Gene
- A15e0
- Outcome
- a unique bande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“One primer, A15, amplified a unique band in both M. floribunda clone 821 and the bulked scab-resistant sample”
Claim · model-written summary
Marker OA15 900 identifies an introgressed fragment that may modify the scab resistance gene block V.
- Taxon
- M. floribundaMalus floribundae0
- Gene
- scab resistance gene block Ve0
- Outcome
- an amplified, introgressed fragmente0
- Qualifier
- likely corresponds to a region of the genome that may serve as a modifiere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This RAPD marker, designated OA15 900 , identifies an amplified, introgressed fragment that likely corresponds to a region of the genome that may serve as a modifier for the scab resistance gene block V, derived from M. floribunda clone 821”