Paper · 2016 · Applied Ecology and Environmental Research

OPTIMIZATION OF THIOUREA LEVEL AT CELLULAR AND WHOLE PLANT LEVEL FOR MAIZE HYBRIDS (ZEA MAYS L.)

T. SANAULLAH

4
claims
10
verbatim quotes
8
citations
3
research areas
✓
Open access

Abstract

Thiourea is a potential plant growth regulator that improves stress tolerance potential in a variety of plants. Exogenous application of thiourea enhanced the growth and yield of plants, but different concentrations are required by different crops therefore optimization of thiourea is needed for each crop plant. These experiments were arranged to screen out optimum level of thiourea for maize hybrids at both whole plant and cellular levels. Two experiments were done, first at whole plant level; two hybrids DK6789 and 33M15 were used. Thirteen levels of thiourea (0, 200, and 400, to 2400 M) were applied through sand application to plants after 15 days of germination, and in second experiment same hybrids were used to investigate optimum level of thiourea for callusing attributes. Immature embryo were cultured on N6 (Chu et al) medium supplemented with 3.99g/L salt, 3% sucrose, 2.5 g/L proline, 0.2g/L casein hydrolysate, 2,4-D 2g/L, AgNO 3 0.015 g/L, and agar 8g/L. Above applied levels of thiourea were supplemented to callus induction medium. Among all applied thiourea levels, 400 M thiourea level was found to be most effective. Thiourea significantly improved in vivo attributes like shoot and root length, fresh and dry weights of shoot and root, leaf area and shoot/root mass ratio. Thiourea was also found very effective in improving in vitro attributes especially improved callus quantity and quality. This could be a great achievement towards better maize genetic transformations for development of resistant crops.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Among the tested thiourea levels, 400 M was the most effective for maize hybrids.

Taxon
maizeZea mayse3
Treatment
400 M thiourea levele1
Outcome
effectivee1
Direction
most effectivee1
Comparator
all applied thiourea levelse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Among all applied thiourea levels, 400 M thiourea level was found to be most effective”

✓ located verbatim in the source · abstract, characters 1057–1143 · show in text ↑
e3

“screen out optimum level of thiourea for maize hybrids”

✓ located verbatim in the source · abstract, characters 349–403 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Thiourea significantly improved shoot and root length and fresh and dry weights of shoot and root in maize hybrids.

Taxon
maizeZea mayse3
Material
DK6789 and 33M15e4
Treatment
Thioureae0
Outcome
shoot and root length, fresh and dry weights of shoot and roote0
Direction
significantly improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Thiourea significantly improved in vivo attributes like shoot and root length, fresh and dry weights of shoot and root, leaf area and shoot/root mass ratio”

✓ located verbatim in the source · abstract, characters 1145–1300 · show in text ↑
e3

“screen out optimum level of thiourea for maize hybrids”

✓ located verbatim in the source · abstract, characters 349–403 · show in text ↑
e4

“two hybrids DK6789 and 33M15 were used”

✓ located verbatim in the source · abstract, characters 500–538 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Thiourea significantly improved leaf area and shoot/root mass ratio in maize hybrids.

Taxon
maizeZea mayse3
Material
DK6789 and 33M15e4
Treatment
Thioureae0
Outcome
leaf area and shoot/root mass ratioe0
Direction
significantly improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Thiourea significantly improved in vivo attributes like shoot and root length, fresh and dry weights of shoot and root, leaf area and shoot/root mass ratio”

✓ located verbatim in the source · abstract, characters 1145–1300 · show in text ↑
e3

“screen out optimum level of thiourea for maize hybrids”

✓ located verbatim in the source · abstract, characters 349–403 · show in text ↑
e4

“two hybrids DK6789 and 33M15 were used”

✓ located verbatim in the source · abstract, characters 500–538 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thiourea improved callus quantity and quality in vitro in maize.

Taxon
maizeZea mayse3
Treatment
Thioureae2
Outcome
callus quantity and qualitye2
Direction
improvede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“Thiourea was also found very effective in improving in vitro attributes especially improved callus quantity and quality”

✓ located verbatim in the source · abstract, characters 1302–1421 · show in text ↑
e3

“screen out optimum level of thiourea for maize hybrids”

✓ located verbatim in the source · abstract, characters 349–403 · show in text ↑

thiourea · growth · Zea mays (maize)Results differ

11 papers: 10 positive, 1 negative or no effect

9 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.