Paper · 2016 · Buletin Plasma Nutfah

In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification

Kurniawan Budiarto; Budi Marwoto; Rudy Soehendi

4
claims
6
verbatim quotes
0
citations
1
research areas
✓
Open access

Abstract

Modification of osmotic pressure of in vitro medium-term can be applied to conserve carnation accessions. This successful method inspired the use of nutrient modification technique to have the same function as far as these concerned. The research was then, conducted to evaluate in vitro media for preserving several carnation accessions in low temperature conditions. The research was carried out at Indonesian Ornamental Crops Research Institute (IOCRI) from January 2007 to March 2008. A randomized completely block design with 25 replications was used to accomplish the combination of two factors. The first factor was six commercial carnation accessions, namely cv. Light Pink Candy, Aicardy, Torpedo, Malaga, President, and Rendezvouz. While the second factor dealt with four formulations of conservation media i.e. ½ MS + DMSO 3% + 4% sucrose, modified hyponex + 4% sucrose, modified hyponex (no sucrose) and ½ MS + 4% sucrose (control). The results showed that medium term in vitro conservation for carnation were successfully conducted up to 10 months using in ½ MS + DMSO 3% + 4% sucrose and modified hyponex + 4% sucrose without any significant differences and decreases in viability when transferred in to shoot induction media. In the absent of sucrose, however, the plantlet survivals were only up to 8 months. No significant variation on viability and number of survival plantlet was observed among the carnation accessions in all media tested.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Carnation accessions remained viable for up to 10 months in half MS medium with DMSO and sucrose.

Treatment
½ MS + DMSO 3% + 4% sucrosee0
Outcome
medium term in vitro conservatione0
Value
10 monthse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus caryophyllus
Dianthus caryophyllus · TaxonDarkone ( talk · contribs ) · CC BY-SA 1.0 · Wikimedia Commons ↗

Verbatim

e0

“medium term in vitro conservation for carnation were successfully conducted up to 10 months using in ½ MS + DMSO 3% + 4% sucrose”

✓ located verbatim in the source · abstract, characters 969–1097 · show in text ↑
Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

Carnation accessions remained viable for up to 10 months in modified hyponex with sucrose.

Treatment
modified hyponex + 4% sucrosee0
Outcome
viabilitye0
Direction
without any significant differences and decreasese0
Value
10 monthse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus caryophyllus
Dianthus caryophyllus · TaxonDarkone ( talk · contribs ) · CC BY-SA 1.0 · Wikimedia Commons ↗

Verbatim

e0

“up to 10 months using in ½ MS + DMSO 3% + 4% sucrose and modified hyponex + 4% sucrose without any significant differences and decreases in viability”

✓ located verbatim in the source · abstract, characters 1045–1194 · show in text ↑
t0

“Carnation”

✓ located verbatim in the source · title, characters 45–54 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Without sucrose, carnation plantlet survival lasted only eight months.

Treatment
absent of sucrosee0
Outcome
plantlet survivalse0
Direction
onlye0
Value
8 monthse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus caryophyllus
Dianthus caryophyllus · TaxonDarkone ( talk · contribs ) · CC BY-SA 1.0 · Wikimedia Commons ↗

Verbatim

e0

“In the absent of sucrose, however, the plantlet survivals were only up to 8 months.”

✓ located verbatim in the source · abstract, characters 1241–1324 · show in text ↑
t0

“Carnation”

✓ located verbatim in the source · title, characters 45–54 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Carnation accessions did not differ significantly in viability or surviving plantlet number across media.

Outcome
viability and number of survival plantlete0
Direction
No significant variatione0
Comparator
carnation accessionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus caryophyllus
Dianthus caryophyllus · TaxonDarkone ( talk · contribs ) · CC BY-SA 1.0 · Wikimedia Commons ↗

Verbatim

e0

“No significant variation on viability and number of survival plantlet was observed among the carnation accessions in all media tested.”

✓ located verbatim in the source · abstract, characters 1325–1459 · show in text ↑

sucrose · growth · Dianthus caryophyllus (carnation)Positive and negative results

16 papers: 15 positive, 1 negative or no effect

14 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.