Abstract
In this study, efficient plant regeneration through somatic embryogenesis is achieved in four cultivars of carnation (Nelson, Sagres, Spirit and Impulse). Embryogenic calli were induced on petal explants only, all the calli established on leaf, sepal, receptacle and style explants were not embryogenic. Embryogenic calli were obtained on Morashige and Skoog basal medium (1962) containing sucrose 9%, 2.0 mg L-1 2,4-dichlorophenoxy acetic acid (2,4-D), 0.2 mg L-1 6- benzyladenine (BA). After transfer of embryogenic calli to growth regulator free MS medium or medium containing low amount of 2, 4-D (0.2-0.4 mm L-1) resulted in successful somatic embryogenesis. A high frequency (80-85%) of Somatic embryo germination and development into normal plantlets was observed on half-strength MS medium supplement with 3% sucrose without growth regulator. About 95% of somatic embryo-derived plantlets were acclimatized in the greenhouse conditions.
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Claims 7
Claim · model-written summary
Embryogenic calli arose only from petal explants.
- Taxon
- Carnation
Dianthus caryophyllust0 - Organ / stage
- petal explantse0
- Outcome
- Embryogenic callie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“Embryogenic calli were induced on petal explants only”
Claim · model-written summary
Calli from leaf, sepal, receptacle and style explants were not embryogenic.
- Taxon
- CarnationDianthus caryophyllust0
- Organ / stage
- leaf, sepal, receptacle and style explantse0
- Outcome
- not embryogenice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“all the calli established on leaf, sepal, receptacle and style explants were not embryogenic”
Claim · model-written summary
The specified MS medium produced embryogenic calli.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- Morashige and Skoog basal medium (1962) containing sucrose 9%, 2.0 mg L-1 2,4-dichlorophenoxy acetic acid (2,4-D), 0.2 mg L-1 6- benzyladenine (BA)e0
- Outcome
- Embryogenic callie0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“Embryogenic calli were obtained on Morashige and Skoog basal medium (1962) containing sucrose 9%, 2.0 mg L-1 2,4-dichlorophenoxy acetic acid (2,4-D), 0.2 mg L-1 6- benzyladenine (BA)”
Claim · model-written summary
Transfer to regulator-free MS medium produced somatic embryos.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- growth regulator free MS mediume0
- Outcome
- successful somatic embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“After transfer of embryogenic calli to growth regulator free MS medium or medium containing low amount of 2, 4-D (0.2-0.4 mm L-1) resulted in successful somatic embryogenesis”
Claim · model-written summary
Transfer to low 2,4-D medium produced somatic embryos.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- medium containing low amount of 2, 4-D (0.2-0.4 mm L-1)e0
- Outcome
- successful somatic embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“After transfer of embryogenic calli to growth regulator free MS medium or medium containing low amount of 2, 4-D (0.2-0.4 mm L-1) resulted in successful somatic embryogenesis”
Claim · model-written summary
On half-strength MS with sucrose, somatic embryos germinated and developed into normal plantlets at 80–85% frequency.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- half-strength MS medium supplement with 3% sucrose without growth regulatore0
- Outcome
- Somatic embryo germination and development into normal plantletse0
- Value
- 80-85%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“A high frequency (80-85%) of Somatic embryo germination and development into normal plantlets was observed on half-strength MS medium supplement with 3% sucrose without growth regulator”
Claim · model-written summary
About 95% of somatic embryo-derived plantlets acclimatized in greenhouse conditions.
- Taxon
- CarnationDianthus caryophyllust0
- Outcome
- somatic embryo-derived plantlets were acclimatizede0
- Value
- About 95%e0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carnation (Dianthus caryophyllus L.)”
e0“About 95% of somatic embryo-derived plantlets were acclimatized in the greenhouse conditions”
The same questions in other papers
sucrose · growth · Dianthus caryophyllus (carnation)Positive and negative results
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- negative
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See all 9 papers →in vitro · growth · Dianthus caryophyllus (carnation)Results agree
15 papers in the same direction as this paper
- positive
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.