Abstract
The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in Dianthus caryophyllus L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.
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Claims 7
Claim · model-written summary
The greatest callus induction occurred with 2 mg/l 2,4-D and 1 mg/l BA.
- Taxon
- Dianthus caryophyllus L.
Dianthus caryophyllust0 - Treatment
- 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA)e0
- Outcome
- callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA).”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
Only TDZ or zeatin media, alone or with NAA or IAA, differentiated calli.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Treatment
- 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA)e0
- Outcome
- differentiate callie0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli.”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
The highest average shoot number occurred with 2 mg/l TDZ and 1 mg/l IAA.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Treatment
- 2 mg/l TDZ and 1 mg/l IAAe0
- Outcome
- average number of shootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA.”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
Leaf and internode explants differed significantly in calli producing shoots.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Organ / stage
- leaf and internodee0
- Outcome
- calli producing shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode.”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
Leaf and internode explants differed significantly in shoots per callus.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Organ / stage
- leaf and internodee0
- Outcome
- number of shoots per calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode.”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
Shoots elongated and multiplied on MS medium with BA and agar.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Treatment
- MS medium supplemented with 1 mg/l BA and solidified with 1% agare0
- Outcome
- shoots were elongated and multipliede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar.”
t0“Dianthus caryophyllus L.”
Claim · model-written summary
Rooted and hardened shoots survived in pots at 76% after six weeks.
- Taxon
- Dianthus caryophyllus L.Dianthus caryophyllust0
- Outcome
- survival successe0
- Value
- 76%e0
- Qualifier
- after six weeks of transfer to the potse0
- Setting
- potse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.”
t0“Dianthus caryophyllus L.”
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.