Abstract
Abstract The (1,3)-β-D-glucan also referred to as callose is a main component of cell walls of higher plants. Many physiological processes are associated with the changes in callose deposition. Callose is synthesised by the callose synthase complex while its degradation is regulated by the hydrolytic enzymes β-1,3-glucanases. The latter one specifically degrade (1,3)-β-D-glucans. This work is aimed to study β-1,3-glucanase activities in the leaves of plants at two leaf stage in two diploids ( Agilops tauschii , Triticum monococcum L.), four tetraploids ( Ae. cylindrica , Ae. triuncialis , T. araraticum , T. dicoccum ) and two hexaploids ( T. aestivum L, T. spelta L.). The leaves were subjected to qualitative and quantitative β-1,3-glucanase activity assays. Our results showed that the total β-1,3-glucanase activities were variable and genotype dependent. No significant correlation between β-1,3-glucanase activities and ploidy level was observed. The gel activity assays revealed a single fraction of ~52 kDa Glu1 that was found in all genotypes. The Glu1 fraction corresponds to a single or two acidic Glu isoforms in dependence on genotype. However, none of the acidic Glu fractions can be assigned as a specific for di-, tetra- or hexaploid genotypes. A single basic GluF isoform was detected and found as present in all genotypes.
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Claims 6
Claim · model-written summary
Total β-1,3-glucanase activity varied by genotype.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- genotypee0
- Gene
- β-1,3-glucanasee0
- Outcome
- total β-1,3-glucanase activitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results showed that the total β-1,3-glucanase activities were variable and genotype dependent.”
t0“Wheat and Relative Species”
Claim · model-written summary
β-1,3-glucanase activity had no significant correlation with ploidy level.
- Taxon
- WheatTriticum aestivumt0
- Material
- ploidy levele0
- Gene
- β-1,3-glucanasee0
- Outcome
- β-1,3-glucanase activitiese0
- Direction
- No significant correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant correlation between β-1,3-glucanase activities and ploidy level was observed.”
t0“Wheat and Relative Species”
Claim · model-written summary
A roughly 52 kDa Glu1 activity fraction occurred in every genotype.
- Taxon
- WheatTriticum aestivumt0
- Gene
- Glu1e0
- Outcome
- single fraction of ~52 kDa Glu1e0
- Value
- ~52 kDae0
- Qualifier
- all genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gel activity assays revealed a single fraction of ~52 kDa Glu1 that was found in all genotypes.”
t0“Wheat and Relative Species”
Claim · model-written summary
The Glu1 fraction corresponded to one or two acidic isoforms depending on genotype.
- Taxon
- WheatTriticum aestivumt0
- Material
- genotypee0
- Gene
- Glu1e0
- Outcome
- single or two acidic Glu isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Glu1 fraction corresponds to a single or two acidic Glu isoforms in dependence on genotype.”
t0“Wheat and Relative Species”
Claim · model-written summary
No acidic Glu fraction was specific to di-, tetra-, or hexaploid genotypes.
- Taxon
- WheatTriticum aestivumt0
- Gene
- Glue0
- Outcome
- none of the acidic Glu fractions can be assigned as a specific for di-, tetra- or hexaploid genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“none of the acidic Glu fractions can be assigned as a specific for di-, tetra- or hexaploid genotypes.”
t0“Wheat and Relative Species”
Claim · model-written summary
A single basic GluF isoform was present in all genotypes.
- Taxon
- WheatTriticum aestivumt0
- Gene
- GluFe0
- Outcome
- single basic GluF isoforme0
- Qualifier
- all genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A single basic GluF isoform was detected and found as present in all genotypes.”
t0“Wheat and Relative Species”