Abstract
Aphloia theiformis (Vahl) Benn. (Flacourtiaceae) is a common species found around regions in the Indian Ocean (Madagascar and Mascareignes Islands) and in Tropical Africa (Mozambique, Tanzanie, and South Africa). In Madagascar, this plant is mainly located in "Les Hautes-Terres" region in the east. In traditional Malagasy medicine, the leaves are mainly used as an infusion to treat fever and stomach aches [1, 2]. Previous phytochemical investigations on the leaves revealed a high concentration of aphloiol (also called mangiferin), a C-glycoside xanthone known for its biological properties [3 – 5]. We demonstrated the anti-ageing properties of this molecule for cosmetic applications [6]. Moreover, from A.theiformis leaves of Madagascar, we developed a very pure extract of 85 – 93% aphloïol (w/w of DM), used as a botanical ingredient for cosmetic products. To obtain the best extraction yield, the selection of the harvested leaves with the highest concentration of aphloïol was necessary5. Thus, the aim of our study was to develop an analytical method for use in Madagascar, to quantify aphloïol and to verify the quality of harvested leaves. The method was achieved by densitometric HPTLC-UV method on analytical plates Si-60F254. The plates eluted with ethylacetate:formic acid:acetic acid:water (100:11:11:26) were developed to a distance of 4 cm (RF aphloïol: 0.53) and then scanned at 265nm. A polynomial regression (Stavitsky-Golay model) was applied between the concentrations of aphloïol (60 to 400 ng/spot) and peak areas. A correlation coefficient (r) of 0.9995 was obtained. The method applied on three leaf samples native to Madagascar showed an average concentration of 24.7% (w/w of DM) aphloïol with a SD of 1.4 in our hydroethanolic extracts, and confirmed the repeatability of the method. The present method on quantification of aphloïol by HPTLC-UV in A.theiformis leaves appeared to be efficient, rapid, and simple, and is now used in Madagascar, to check the quality of the leaves after a harvest.
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Claims 5
Claim · model-written summary
A pure extract developed from Madagascar leaves contained 85–93% aphloïol.
- Taxon
- A.theiformis
Aphloia theiformise0 - Organ / stage
- leavese0
- Outcome
- aphloïole0
- Value
- 85 – 93%e0
- Setting
- Madagascare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from A.theiformis leaves of Madagascar, we developed a very pure extract of 85 – 93% aphloïol (w/w of DM)”
Claim · model-written summary
The HPTLC-UV method had a correlation coefficient of 0.9995 over the tested aphloïol concentrations.
- Taxon
- Aphloia theiformisAphloia theiformist0
- Outcome
- correlation coefficient (r)e0
- Value
- 0.9995e0
- Qualifier
- 60 to 400 ng/spote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A polynomial regression (Stavitsky-Golay model) was applied between the concentrations of aphloïol (60 to 400 ng/spot) and peak areas. A correlation coefficient (r) of 0.9995 was obtained.”
t0“Aphloia theiformis”
Claim · model-written summary
The method measured mean aphloïol concentration of 24.7% in three Madagascar leaf samples.
- Taxon
- Aphloia theiformisAphloia theiformist0
- Organ / stage
- leafe0
- Outcome
- average concentration of 24.7% (w/w of DM) aphloïole0
- Value
- 24.7% (w/w of DM)e0
- Qualifier
- SD of 1.4e0
- Setting
- Madagascare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The method applied on three leaf samples native to Madagascar showed an average concentration of 24.7% (w/w of DM) aphloïol with a SD of 1.4 in our hydroethanolic extracts, and confirmed the repeatability of the method.”
t0“Aphloia theiformis”
Claim · model-written summary
Application to three leaf samples confirmed repeatability of the method.
- Taxon
- Aphloia theiformisAphloia theiformist0
- Outcome
- repeatability of the methode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confirmed the repeatability of the method.”
t0“Aphloia theiformis”
Claim · model-written summary
The authors found the leaf quantification method efficient, rapid, and simple.
- Taxon
- A.theiformisAphloia theiformise0
- Outcome
- efficient, rapid, and simplee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The present method on quantification of aphloïol by HPTLC-UV in A.theiformis leaves appeared to be efficient, rapid, and simple”