Abstract
The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL L-1), pH adjusted to 5.5 ± 0.5 and gelated with 4GL-1 agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.
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Claims 9
Claim · model-written summary
Potassium silicate elongated the hypodermis.
Not reportedComparator
Verbatim
t0“Catasetum x apolloi”
e0“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate thickened the mesophyll.
Not reportedComparator
Verbatim
t0“Catasetum x apolloi”
e0“thicker mesophyll”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate made the midrib more prominent.
Not reportedComparator
Verbatim
t0“Catasetum x apolloi”
e0“more prominent midrib”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate produced elliptical guard cells.
Not reportedComparatorDirection
Verbatim
t0“Catasetum x apolloi”
e0“elipptical guard cells”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate induced an epistomatal chamber.
Not reportedComparatorDirection
Verbatim
t0“Catasetum x apolloi”
e0“formation of epistomatal chamber”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate lowered stomatal density.
Not reportedComparator
Verbatim
t0“Catasetum x apolloi”
e0“lower stomatal density”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Potassium silicate lowered stomatal equatorial and polar diameters.
Not reportedComparator
Verbatim
t0“Catasetum x apolloi”
e0“stomatal with lower equatorial and polar diameters”
e1“Applications in K2SiO4 alternative medium provided the following: elongation of the hypodermis”
Claim · model-written summary
Lower light intensities favored acclimatization.
Verbatim
t0“Catasetum x apolloi”
e0“The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses”
Claim · model-written summary
Lower potassium silicate doses favored acclimatization.
Verbatim
t0“Catasetum x apolloi”
e0“The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses”