Abstract
In the breeding of early maturing peach and nectarine cultivars, the use of embryo rescue techniques is invaluable since the embryos of these materials are not mature by the time the fruit is mature. Thus the seed does not germinate under normal stratification/germination procedures. Peach embryos between 3 and 6 mm in length were cultured in ovule on WPM under treatments that varied in sucrose levels (4% and 6%),the use of vitamins (casein hydrosylate, ascorbic acid, and panthotenic acid) and hormones (BAP and GA 3 ), and the duration of the treatment (7, 14, 21, and 28 days). A five-seed sample was measured for length, fresh weight, and dry weight before and after treatment. The treated embryos were then cultured in a embryo rescue media (WPM, 3% sucrose) at 5 °C for 45 days in the dark and then brought out to room temperature under lights (12-h day). After 2 to 4 weeks the embryos were rated for germination, size, and root number. Embryo perforation treatment resulted in larger embryos but not in better germination than the smaller unperforated embryos. Among the embryos cultured without perforation, the addition of vitamins and hormones caused the production of plants with more roots and shoots but not necessarily with better survival. Both levels of sucrose worked reasonable well. As the time of in ovule treatment increased, embryo growth and plant survival was enhanced.
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Claims 6
Claim · model-written summary
Perforation produced larger peach embryos.
- Taxon
- Peach
Prunus persicat0 - Treatment
- Embryo perforation treatmente0
- Outcome
- embryose0
- Direction
- largere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryo perforation treatment resulted in larger embryos”
t0“Peach”
Claim · model-written summary
Perforation did not improve germination over unperforated embryos.
- Taxon
- PeachPrunus persicat0
- Treatment
- Embryo perforation treatmente0
- Outcome
- germinatione0
- Direction
- not in bettere0
- Qualifier
- smallere0
- Comparator
- unperforated embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryo perforation treatment resulted in larger embryos but not in better germination than the smaller unperforated embryos”
t0“Peach”
Claim · model-written summary
Added vitamins and hormones produced more roots and shoots in plants from unperforated embryos.
- Taxon
- PeachPrunus persicat0
- Treatment
- addition of vitamins and hormonese0
- Outcome
- roots and shootse0
- Direction
- moree0
- Qualifier
- without perforatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the embryos cultured without perforation, the addition of vitamins and hormones caused the production of plants with more roots and shoots”
t0“Peach”
Claim · model-written summary
The added vitamins and hormones did not necessarily improve survival.
- Taxon
- PeachPrunus persicat0
- Treatment
- addition of vitamins and hormonese0
- Outcome
- survivale0
- Direction
- not necessarily with bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of vitamins and hormones caused the production of plants with more roots and shoots but not necessarily with better survival”
t0“Peach”
Claim · model-written summary
Both tested sucrose levels worked reasonably well.
- Taxon
- PeachPrunus persicat0
- Treatment
- Both levels of sucrosee0
- Outcome
- worked reasonable welle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both levels of sucrose worked reasonable well”
t0“Peach”
Claim · model-written summary
Longer in-ovule treatment enhanced embryo growth and plant survival.
- Taxon
- PeachPrunus persicat0
- Treatment
- time of in ovule treatmente0
- Outcome
- embryo growth and plant survivale0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As the time of in ovule treatment increased, embryo growth and plant survival was enhanced”
t0“Peach”