Abstract
The Role of Growth Regulator in Tissue Culture Plant Propagation. Endang G. Lestari. In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction. Cytokinin and auxin are two prominent growth regulator. Cytokinin consists of BA (benzil adenin), kinetin (furfuril amino purin), 2-Ip (dimethyl allyl amino purin), and zeatin. While auksin covers IAA (indone acetic acid), NAA (napthalene acetic acid), IBA (indole butiric acid) 2.4-D (2.4- dicholophenoxy acetic acid), dicamba (3,6 dicloro-O-anisic acid), and picloram (4-amino 3,5,6-tricloropicolinic acid). The emphasis of plant growth purposes decide the use of growth regulator. Cytokinin is applied mainly for the purpose of shoot, while auxin is mainly used for the purpose of root and callus. The application of growth regulator application is varied, depending on the genotype and physiological condition of the plant. The existence of a certain growth regulating substances can enhance growth regulator activity of other substances. The type and concentration of the appropriate growth regulators for each plant is not the same because it depends on the genotype and physiological condition of plant tissue. However so often both are frequently required depend on the ratio/ratio of auxin cytokines or vice versa. The existence of a certain growth regulating substances can enhance growth regulator activity of other substances. The type and concentration of the appropriate growth regulators for each plant is not the same because it depends on the genotype and physiological condition of plant tissue. For the propagation, multiple and adventive shoots along with embriosomatic formation could be applied. The seedling is obtained from one somatic cell. Here, strong auxin, such as dicamba and picloram 2.4-D, is utilized for callus production. For this reason, seedling per unit could be produced more than that of organogenesis.
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Claims 5
Claim · model-written summary
Growth regulators control root and shoot development and callus induction in plant tissue culture.
Verbatim
e1“In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction”
Claim · model-written summary
Cytokinin is applied mainly for shoot formation, whereas auxin is mainly used for root and callus formation.
Verbatim
e1“Cytokinin is applied mainly for the purpose of shoot, while auxin is mainly used for the purpose of root and callus”
e2“In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction”
Claim · model-written summary
The application of growth regulators varies depending on the genotype and physiological condition of the plant.
Verbatim
e1“The application of growth regulator application is varied, depending on the genotype and physiological condition of the plant”
Claim · model-written summary
Strong auxins such as dicamba, picloram and 2.4-D are used for callus production.
Verbatim
e1“strong auxin, such as dicamba and picloram 2.4-D, is utilized for callus production”
e2“In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction”
Claim · model-written summary
Via callus and somatic embryogenesis, more seedlings per unit can be produced than by organogenesis.
Verbatim
e1“seedling per unit could be produced more than that of organogenesis”
e2“In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction”