Abstract
This study was conducted with the aim of determining the utilization of embryo culture in tomato. Reciprocal crosses were carried out using the tomato (L. esculentum) cultivars "Rio Grande", "H 2274", "Tivoli", "Ontario 7710", "P.19" and wild tomato species L. pimpinellifolium and L. peruvianum. No problem was encountered with the crosses carried out by using L. pimpinellifolium; 24 days old embryos obtained from the crosses in which L. pimpinellifolium was used as male or female parent were isolated and cultured on two different nutrient media. At the end of the trials, the male or female parent used, nutrient medium, and the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio". Some problems were encountered in the crosses with L. peruvianum; no fruits could be obtained when L. peruvianum was the female parent due to unilateral incompatibility, whereas fruits were obtained when this wild species was the male parent, however, the embryos in these fruits were determined to be aborted. This type of fruits had no embryos to be excised even on the 40(th) day after pollination, and these seeds were not able to germinate even when they were put on suitable nutrient media. Therefore, in L. esculentum x L. peruvianum crosses, an attempt was made to obtain plantlets via callus formation from the seeds with aborted embryos as an alternative to embryo culture.
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Claims 12
Claim · model-written summary
Crosses using L. pimpinellifolium encountered no problem.
Verbatim
e0“No problem was encountered with the crosses carried out by using L. pimpinellifolium”
Claim · model-written summary
Male or female parent affected embryo survival ratio in L. pimpinellifolium crosses.
Not reportedDirection
Verbatim
e0“the male or female parent used, nutrient medium, and the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
Nutrient medium affected embryo survival ratio in L. pimpinellifolium crosses.
Not reportedDirection
Verbatim
e0“the male or female parent used, nutrient medium, and the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
Parent by medium interaction affected embryo survival ratio.
- Taxon
- L. pimpinellifoliume1
- Treatment
- interaction of these two factorse0
- Outcome
- "Survival Ratio"e0
- Qualifier
- interaction of these two factorse0
Not reportedDirection
Verbatim
e0“the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
Male or female parent affected whole-plant formation ratio.
Not reportedDirection
Verbatim
e0“the male or female parent used, nutrient medium, and the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
Nutrient medium affected whole-plant formation ratio.
Not reportedDirection
Verbatim
e0“the male or female parent used, nutrient medium, and the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
Parent by medium interaction affected whole-plant formation ratio.
- Taxon
- L. pimpinellifoliume1
- Treatment
- interaction of these two factorse0
- Outcome
- "Whole Plant Forming Ratio"e0
Not reportedDirection
Verbatim
e0“the interaction of these two factors were determined to affect the "Survival Ratio" and "Whole Plant Forming Ratio".”
e1“L. pimpinellifolium was used as male or female parent”
Claim · model-written summary
No fruit formed when L. peruvianum was the female parent because of unilateral incompatibility.
Verbatim
e0“no fruits could be obtained when L. peruvianum was the female parent due to unilateral incompatibility”
Claim · model-written summary
Fruit formed when L. peruvianum was the male parent.
Verbatim
e0“fruits were obtained when this wild species was the male parent”
e1“crosses with L. peruvianum”
Claim · model-written summary
Embryos in those fruits aborted.
Verbatim
e0“the embryos in these fruits were determined to be aborted”
e1“crosses with L. peruvianum”
Claim · model-written summary
The fruits had no excisable embryos even 40 days after pollination.
Verbatim
e0“This type of fruits had no embryos to be excised even on the 40(th) day after pollination”
e1“crosses with L. peruvianum”
Claim · model-written summary
Seeds from those fruits could not germinate on suitable medium.
Verbatim
e0“these seeds were not able to germinate even when they were put on suitable nutrient media”
e1“crosses with L. peruvianum”