Abstract
Early responsive to dehydration (ERD) genes can be induced rapidly to counter some abiotic stresses, such as drought, low temperatures or high salinities. Here, we report an ERD gene (VaERD15) related to cold-tolerance from Chinese wild Vitis amurensis accession ‘Heilongjiang seedling’. The full-length of VaERD15 cDNA is 685 bp including 66 bp 5′-untranslated region (UTR), 196 bp 3′ -UTR region and a 423 bp ORF (open reading frame) encoding 140 amino acids. The VaERD15 protein shares a high amino acid sequence similarity with ERD15 of Arabidopsis thaliana. In our study, VaERD15 proved to have a nucleic localization function and a transcriptional activation function. Semi-quantitative PCR and Western blot analyses showed that VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis accession ‘Heilongjiang seedling’ plants but the expression levels increased after low-temperature treatment. We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0 and carried out a cold treatment assay. The qRT-PCR and western blot analyses showed that with the increasing duration of cold treatment, the expression both in gene and protein levels was raised continuously in transgenic plants, and almost nothing was detected in the wild type arabidopsis. Moreover, the VaERD15 over-expression plants showed higher cold tolerance and the accumulations of proline, soluble sugars and proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase and catalase). Lower levels of relative ion leakage also occurred under cold stress. Together, our results indicate that the transcription factor VaERD15 was induced by cold stress and that it also enhanced cold tolerance.
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Claims 11
Claim · model-written summary
VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis.
- Taxon
- V. amurensis
Vitis amurensise1 - Material
- Heilongjiang seedlinge1
- Organ / stage
- young leaves, stems and rootse1
- Gene
- VaERD15e1
- Outcome
- constitutively expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis accession ‘Heilongjiang seedling’ plants but the expression levels increased after low-temperature treatment”
Claim · model-written summary
VaERD15 expression levels increased after low-temperature treatment in V. amurensis.
- Taxon
- V. amurensisVitis amurensise1
- Material
- Heilongjiang seedlinge1
- Treatment
- low-temperature treatmente1
- Gene
- VaERD15e1
- Outcome
- expression levelse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis accession ‘Heilongjiang seedling’ plants but the expression levels increased after low-temperature treatment”
Claim · model-written summary
The VaERD15 cDNA is 685 bp full-length.
- Taxon
- Vitis amurensisVitis amurensise0
- Gene
- VaERD15e2
- Outcome
- full-length of VaERD15 cDNAe2
- Value
- 685 bpe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“related to cold-tolerance from Chinese wild Vitis amurensis accession”
e2“The full-length of VaERD15 cDNA is 685 bp”
Claim · model-written summary
The VaERD15 protein has a nucleic localization function and a transcriptional activation function.
- Taxon
- Vitis amurensisVitis amurensise0
- Gene
- VaERD15e4
- Outcome
- nucleic localization function and a transcriptional activation functione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“related to cold-tolerance from Chinese wild Vitis amurensis accession”
e4“VaERD15 proved to have a nucleic localization function and a transcriptional activation function”
Claim · model-written summary
The VaERD15 protein shares high amino acid sequence similarity with ERD15 of Arabidopsis thaliana.
- Taxon
- Vitis amurensisVitis amurensise0
- Gene
- VaERD15e3
- Outcome
- amino acid sequence similaritye3
- Comparator
- ERD15 of Arabidopsis thalianae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“related to cold-tolerance from Chinese wild Vitis amurensis accession”
e3“The VaERD15 protein shares a high amino acid sequence similarity with ERD15 of Arabidopsis thaliana”
Claim · model-written summary
VaERD15 gene and protein expression in transgenic Arabidopsis rose continuously with the duration of cold treatment, while the wild type showed almost none.
- Taxon
- Arabidopsis
Arabidopsise2 - Material
- transgenic plantse1
- Treatment
- cold treatmente1
- Gene
- VaERD15e2
- Outcome
- expression both in gene and protein levelse1
- Direction
- raisede1
- Comparator
- wild type arabidopsise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“with the increasing duration of cold treatment, the expression both in gene and protein levels was raised continuously in transgenic plants, and almost nothing was detected in the wild type arabidopsis”
e2“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
Claim · model-written summary
VaERD15 over-expression plants showed higher cold tolerance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- VaERD15 over-expressione2
- Outcome
- cold tolerancee2
- Direction
- highere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
e2“the VaERD15 over-expression plants showed higher cold tolerance and the accumulations of proline, soluble sugars and proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase and catalase)”
Claim · model-written summary
VaERD15 over-expression plants showed increased accumulation of proline, soluble sugars and proteins.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- VaERD15 over-expressione2
- Outcome
- proline, soluble sugars and proteinse2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
e2“the VaERD15 over-expression plants showed higher cold tolerance and the accumulations of proline, soluble sugars and proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase and catalase)”
Claim · model-written summary
VaERD15 over-expression plants showed increased superoxide dismutase, peroxidase and catalase.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- VaERD15 over-expressione2
- Outcome
- superoxide dismutase, peroxidase and catalasee2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
e2“the VaERD15 over-expression plants showed higher cold tolerance and the accumulations of proline, soluble sugars and proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase and catalase)”
Claim · model-written summary
VaERD15 over-expression plants showed increased malondialdehyde.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- VaERD15 over-expressione2
- Outcome
- malondialdehydee2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
e2“the VaERD15 over-expression plants showed higher cold tolerance and the accumulations of proline, soluble sugars and proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase and catalase)”
Claim · model-written summary
Relative ion leakage was lower under cold stress in the VaERD15 plants.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- cold stresse3
- Outcome
- relative ion leakagee3
- Direction
- Lowere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We also generated the over-expression transgenic line of VaERD15 in Arabidopsis Col-0”
e3“Lower levels of relative ion leakage also occurred under cold stress”