Abstract
Abstract Apposite development of anther and its dehiscence are important for the reproductive success of the flowering plants. Recently, bHLH142 , a bHLH transcription factor encoding gene of rice has been found to show anther-specific expression and mutant analyses suggest its functions in regulating tapetum differentiation and degeneration during anther development. However, our study on protein level expression and gain-of-function phenotype revealed novel aspects of its regulation and function during anther development. Temporally dissimilar pattern of bHLH142 transcript and polypeptide accumulation suggested regulation of its expression beyond transcriptional level. Overexpression of bHLH142 in transgenic rice resulted in indehiscent anthers and aborted pollen grains. Defects in septum and stomium rupture caused anther indehiscence while pollen abortion phenotype attributed to abnormal degeneration of the tapetum. Furthermore, RNA-Seq-based transcriptome analysis of tetrad and mature pollen stage anthers of wild type and bHLH142 OE plants suggested that it might regulate carbohydrate and lipid metabolism, cell wall modification, reactive oxygen species (ROS) homeostasis and cell death-related genes during rice anther development. Thus, bHLH142 is an anther-specific gene whose expression is regulated at transcriptional and post-transcriptional/translational levels. It plays a role in pollen maturation and anther dehiscence by regulating expression of various metabolic pathways-related genes.
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Claims 6
Claim · model-written summary
bHLH142 transcript and protein accumulation had temporally different patterns.
- Taxon
- rice
Oryza sativat0 - Gene
- bHLH142e0
- Outcome
- Temporally dissimilar pattern of bHLH142 transcript and polypeptide accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Temporally dissimilar pattern of bHLH142 transcript and polypeptide accumulation”
t0“rice”
Claim · model-written summary
bHLH142 overexpression produced indehiscent anthers.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of bHLH142e0
- Gene
- bHLH142e0
- Outcome
- indehiscent antherse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of bHLH142 in transgenic rice resulted in indehiscent anthers and aborted pollen grains.”
Claim · model-written summary
bHLH142 overexpression produced aborted pollen grains.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of bHLH142e0
- Gene
- bHLH142e0
- Outcome
- aborted pollen grainse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of bHLH142 in transgenic rice resulted in indehiscent anthers and aborted pollen grains.”
Claim · model-written summary
Failed septum and stomium rupture caused anther indehiscence.
- Taxon
- riceOryza sativat0
- Outcome
- anther indehiscencee0
- Qualifier
- Defects in septum and stomium rupturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Defects in septum and stomium rupture caused anther indehiscence”
t0“rice”
Claim · model-written summary
Abnormal tapetum degeneration was attributed as the cause of pollen abortion.
- Taxon
- riceOryza sativat0
- Organ / stage
- tapetume0
- Outcome
- pollen abortion phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pollen abortion phenotype attributed to abnormal degeneration of the tapetum”
t0“rice”
Claim · model-written summary
Transcriptome analysis suggested bHLH142 may regulate carbohydrate and lipid metabolism, cell wall modification, ROS homeostasis, and cell death genes.
- Taxon
- riceOryza sativae0
- Organ / stage
- anthere0
- Gene
- bHLH142t0
- Outcome
- carbohydrate and lipid metabolism, cell wall modification, reactive oxygen species (ROS) homeostasis and cell death-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it might regulate carbohydrate and lipid metabolism, cell wall modification, reactive oxygen species (ROS) homeostasis and cell death-related genes during rice anther development.”
t0“bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice”