Abstract
This study aimed to dissect the function of the Isochorismatase Hydrolase (ICSH1) gene in Verticillium dahliae’s pathogenesis on potato. VdICSH1 was up-regulated in V. dahliae after induction with extracts from potato tissues. Its expression increased more in response to root extracts than to leaf and stem extracts. However, such expression in response to root extracts was not significantly different in the highly and weakly aggressive isolates tested. During infection of detached potato leaves, VdICSH1 expression increased significantly in the highly aggressive isolate compared to the weakly aggressive one. We generated icsh1 mutants from a highly aggressive isolate of V. dahliae and compared their pathogenicity with that of the original wild-type strain. The analysis showed that this gene is required for full virulence of V. dahliae on potatoes. When we previously found differential accumulation of ICSH1 protein in favor of the highly aggressive isolate, as opposed to the weakly aggressive one, we had hypothesized that ICSH would interfere with the host’s defense SA-based signaling. Here, we measured the accumulation of both salicylic acid (SA) and jasmonic acid (JA) in potato plants inoculated with an icsh1 mutant in comparison with the wild-type strain. The higher accumulation of bound SA in response to the icsh1 mutant compared to the wild type confirms the hypothesis that ICSH1 interferes with SA. However, the different trends in SA and JA accumulation in potato in the roots and in the stems at the early infection stages compared to the leaves at later stages indicate that they are both associated to potato defenses against V. dahliae. The expression of members of the isochorismatase family in the icsh1 mutants compensate that of ICSH1 transcripts, but this compensation disappears in presence of the potato leaf extracts. This study indicates ICSH1’s involvement in V. dahliae’s pathogenicity and provides more insight into its alteration of the SA/JA defense signaling’s networking.
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Claims 8
Claim · model-written summary
VdICSH1 expression was up-regulated in V. dahliae after induction with extracts from potato tissues.
- Taxon
- V. dahliae
Verticillium dahliaee0 - Treatment
- extracts from potato tissuese0
- Gene
- VdICSH1e0
- Outcome
- VdICSH1 was up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdICSH1 was up-regulated in V. dahliae after induction with extracts from potato tissues”
Claim · model-written summary
VdICSH1 expression increased more in response to potato root extracts than to leaf and stem extracts.
- Taxon
- V. dahliaeVerticillium dahliaee0
- Treatment
- root extractse1
- Outcome
- expression increased moree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdICSH1 was up-regulated in V. dahliae”
e1“Its expression increased more in response to root extracts than to leaf and stem extracts”
Claim · model-written summary
VdICSH1 expression in response to root extracts did not differ significantly between the highly and weakly aggressive isolates tested.
- Taxon
- V. dahliaeVerticillium dahliaee0
- Treatment
- root extractse1
- Outcome
- expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdICSH1 was up-regulated in V. dahliae”
e1“such expression in response to root extracts was not significantly different in the highly and weakly aggressive isolates tested”
Claim · model-written summary
During infection of detached potato leaves, VdICSH1 expression increased significantly in the highly aggressive isolate compared with the weakly aggressive one.
- Taxon
- potato
Solanum tuberosume0 - Organ / stage
- leavese0
- Gene
- VdICSH1e0
- Outcome
- VdICSH1 expression increased significantlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During infection of detached potato leaves, VdICSH1 expression increased significantly in the highly aggressive isolate compared to the weakly aggressive one”
Claim · model-written summary
icsh1 mutants of V. dahliae were less virulent on potato than the wild-type strain; the gene is required for full virulence.
- Taxon
- potatoesSolanum tuberosume1
- Treatment
- icsh1 mutantse0
- Interactor
- V. dahliaeVerticillium dahliaee1pathogen
- Outcome
- full virulencee1
- Direction
- decreaseinferrede1
- Comparator
- original wild-type straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We generated icsh1 mutants from a highly aggressive isolate of V. dahliae and compared their pathogenicity with that of the original wild-type strain”
e1“this gene is required for full virulence of V. dahliae on potatoes”
Claim · model-written summary
Potato plants inoculated with the icsh1 mutant accumulated more bound SA than those inoculated with the wild-type strain.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- icsh1 mutante1
- Outcome
- accumulation of bound SAe1
- Direction
- highere1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we measured the accumulation of both salicylic acid (SA) and jasmonic acid (JA) in potato plants inoculated with an icsh1 mutant in comparison with the wild-type strain”
e1“The higher accumulation of bound SA in response to the icsh1 mutant compared to the wild type”
Claim · model-written summary
SA and JA accumulation in potato followed different trends in roots and stems at early infection stages versus leaves at later stages, indicating both are associated with potato defenses against V. dahliae.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- rootse0
- Outcome
- SA and JA accumulatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the different trends in SA and JA accumulation in potato in the roots and in the stems at the early infection stages compared to the leaves at later stages”
Claim · model-written summary
Expression of isochorismatase family members in icsh1 mutants compensates for ICSH1 transcripts, but this compensation disappears in the presence of potato leaf extracts.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- expression of members of the isochorismatase familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of members of the isochorismatase family in the icsh1 mutants compensate that of ICSH1 transcripts, but this compensation disappears in presence of the potato leaf extracts”