Abstract
The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%) and Q117 (82.83 ± 1.69%). Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively. Cell suspension cultures were established by culturing embryogenic calli in liquid medium showed well established suspension cultures with fever cell aggregates. There was negligible cell division during initial 2 days of incubation and cell count increased rapidly between 2 and 8 days. Further incubation beyond 8 days resulted in a decrease in cell viability. Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture. The clonal fidelity of in vitro regenerated plants was assessed by using RAPD and ISSR markers. Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively. However, in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found. These results confirmed that somaclonal variation occurs during the process of indirect organogenesis and RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.
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Claims 16
Claim · model-written summary
Medium VI yielded high callus formation in Co 86032 at 79.66 ± 0.44%.
- Taxon
- Saccharum officinarum L.
Saccharum officinarumt0 - Material
- Co 86032e0
- Treatment
- medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffere0
- Outcome
- calluse0
- Direction
- highe0
- Value
- 79.66 ± 0.44%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%)”
t0“Saccharum officinarum L.”
Claim · model-written summary
Medium VI yielded high callus formation in Q117 at 82.83 ± 1.69%.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Q117e0
- Treatment
- medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffere0
- Outcome
- calluse0
- Direction
- highe0
- Value
- 82.83 ± 1.69%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%) and Q117 (82.83 ± 1.69%)”
t0“Saccharum officinarum L.”
Claim · model-written summary
Adding 2.5% PEG 8000 increased somatic embryogenesis in Co 86032 to 54.66 ± 1.76% versus 24.33 ± 1.76% in control.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Co 86032e0
- Treatment
- Addition of PEG 8000 at 2.5%e0
- Outcome
- somatic embryogenesise0
- Value
- 54.66 ± 1.76%e0
- Qualifier
- 24.33 ± 1.76%e0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Adding 2.5% PEG 8000 increased somatic embryogenesis in Q117 to 66.66 ± 2.60% versus 27.33 ± 2.73% in control.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Q117e0
- Treatment
- Addition of PEG 8000 at 2.5%e0
- Outcome
- somatic embryogenesise0
- Value
- 66.66 ± 2.60%e0
- Qualifier
- 27.33 ± 2.73%e0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Liquid culture of embryogenic calli established suspension cultures with fewer cell aggregates.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Treatment
- culturing embryogenic calli in liquid mediume0
- Outcome
- well established suspension cultures with fever cell aggregatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell suspension cultures were established by culturing embryogenic calli in liquid medium showed well established suspension cultures with fever cell aggregates.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Cell division was negligible during the initial two days of incubation.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Outcome
- cell divisione0
- Direction
- negligiblee0
- Qualifier
- initial 2 days of incubatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was negligible cell division during initial 2 days of incubation”
t0“Saccharum officinarum L.”
Claim · model-written summary
Cell count rose rapidly between days 2 and 8.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Outcome
- cell counte0
- Direction
- increased rapidlye0
- Qualifier
- between 2 and 8 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell count increased rapidly between 2 and 8 days”
t0“Saccharum officinarum L.”
Claim · model-written summary
Incubation beyond eight days reduced cell viability.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Outcome
- cell viabilitye0
- Direction
- decreasee0
- Qualifier
- beyond 8 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further incubation beyond 8 days resulted in a decrease in cell viability.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Q117 showed enhanced callus proliferation from suspension culture.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Q117e0
- Treatment
- cell suspension culturee0
- Outcome
- callus proliferatione0
- Direction
- Enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Co 86032 showed enhanced shoot regeneration from suspension culture.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Co 86032e0
- Treatment
- cell suspension culturee0
- Outcome
- shoot regeneratione0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture.”
t0“Saccharum officinarum L.”
Claim · model-written summary
RAPD markers identified 90.48% true-to-type regenerated Co 86032 plantlets.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Co 86032e0
- Outcome
- true-to-type regenerated plantletse0
- Value
- 90.48e0
- Qualifier
- ten RAPD markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively.”
t0“Saccharum officinarum L.”
Claim · model-written summary
RAPD markers identified 86.95% true-to-type regenerated Q117 plantlets.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Q117e0
- Outcome
- true-to-type regenerated plantletse0
- Value
- 86.95%e0
- Qualifier
- ten RAPD markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively.”
t0“Saccharum officinarum L.”
Claim · model-written summary
ISSR markers found no polymorphism in regenerated Co 86032 plants.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Co 86032e0
- Outcome
- did not show any polymorphisme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found.”
t0“Saccharum officinarum L.”
Claim · model-written summary
ISSR markers found 92.18% true-to-type Q117 plantlets.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Material
- Q117e0
- Outcome
- true-to-type plantletse0
- Value
- 92.18%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found.”
t0“Saccharum officinarum L.”
Claim · model-written summary
Somaclonal variation occurred during indirect organogenesis.
- Taxon
- Saccharum officinarum L.Saccharum officinarumt0
- Outcome
- somaclonal variatione0
- Qualifier
- during the process of indirect organogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somaclonal variation occurs during the process of indirect organogenesis”
t0“Saccharum officinarum L.”
Claim · model-written summary
RAPD and ISSR molecular analysis was suitable for early detection of variation in sugarcane.
Verbatim
e0“RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.”
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